Kirschmeier P, Gattoni-Celli S, Dina D, Weinstein I B
Proc Natl Acad Sci U S A. 1982 May;79(9):2773-7. doi: 10.1073/pnas.79.9.2773.
Carcinogen- or radiation-transformed C3H 10T1/2 murine fibroblasts transcribe a set of poly(A)+RNAs that contain sequences homologous to the long terminal repeat (LTR) sequence of Moloney murine sarcoma virus. These LTR-containing RNAs consist of a series of discrete bands ranging in size from about 38 to 18 S. The higher molecular weight molecules (30-38 S) in this set of RNAs also contain sequences homologous to the gag, pol, and env genes of a murine leukemia virus. A 24S RNA contains sequences homologous to the env gene of murine leukemia virus. A 20S and an 18S RNA also share homology with the LTR probe but fail to hybridize to the gag, pol, or env probes or to a probe for the U3 region of the LTR sequence. Thus, the latter transcripts do not appear to arise from a known endogenous murine leukemia virus genome. Although this entire set of RNAs is absent from normal C3H 10T1/2 cells (or is present at an extremely low level), these RNAs are induced by BrdUrd or 5-azacytidine. The presence of these RNAs may provide highly sensitive molecular markers of transformation of murine cells.
致癌物或辐射转化的C3H 10T1/2小鼠成纤维细胞转录出一组多聚腺苷酸加尾（poly(A)+）RNA，这些RNA包含与莫洛尼鼠肉瘤病毒长末端重复序列（LTR）同源的序列。这些含LTR的RNA由一系列大小约为38S至18S的离散条带组成。这组RNA中分子量较高的分子（30 - 38S）还包含与鼠白血病病毒的gag、pol和env基因同源的序列。一种24S RNA包含与鼠白血病病毒env基因同源的序列。一种20S和一种18S RNA也与LTR探针有同源性，但不能与gag、pol或env探针杂交，也不能与LTR序列U3区域的探针杂交。因此，后一种转录本似乎并非来自已知的内源性鼠白血病病毒基因组。尽管正常C3H 10T1/2细胞中不存在这整套RNA（或含量极低），但这些RNA可被溴脱氧尿苷（BrdUrd）或5 - 氮杂胞苷诱导产生。这些RNA的存在可能为小鼠细胞转化提供高度敏感的分子标志物。
