Dhar R, McClements W L, Enquist L W, Vande Woude G F
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41. doi: 10.1073/pnas.77.7.3937.
Integrated Moloney murine sarcoma provirus (MSV) has direct terminal repeat sequences (TRS). We determined the nucleotide sequence of both 588-base-pair TRS elements and the adjacent host and viral junctions of an integrated MSV cloned in bacteriophage lambda. Sequences were identified corresponding to the tRNAPro primer binding site in genomic RNA and the reverse-transcribed minus strong stop DNA. Each 588-base-pair repeat contains putative sites for promoting RNA synthesis and RNA polyadenylylation. The first and last 11 nucleotides of the TRS are inverted with respect to each other, and the same four-nucleotide host sequence is found bracketing integrated MSV. Some similarities of TRS and prokaryotic insertion sequence elements are discussed.
整合型莫洛尼鼠肉瘤前病毒(MSV)具有直接末端重复序列(TRS)。我们测定了克隆于噬菌体λ中的整合型MSV的两个588碱基对TRS元件以及相邻宿主与病毒连接区的核苷酸序列。鉴定出了与基因组RNA中的tRNAPro引物结合位点以及逆转录的负链强终止DNA相对应的序列。每个588碱基对的重复序列都包含促进RNA合成和RNA聚腺苷酸化的假定位点。TRS的第一个和最后11个核苷酸彼此反向,并且在整合型MSV的两侧发现了相同的四核苷酸宿主序列。文中还讨论了TRS与原核插入序列元件的一些相似性。