Rat hybridoma antibodies against canine parvovirus.

Hybrid cells have been produced by fusion between rat myeloma cells IR983F and spleen cells from a LOU rat immunized with canine enteritis parvovirus. Four stable cell lines were selected each secreting a monoclonal antibody directed against this virus. By indirect immunofluorescence, the antigenic determinants recognized by these antibodies were found to be present not only in canine parvovirus infected cells but also in cells infected by the related parvoviruses of mink enteritis and feline panleukopenia. Two of the monoclonal antibodies inhibit hemagglutination by the canine virus and neutralize its infectivity. Hybrid cells were transplanted into histocompatible animals, and large amounts of the antibodies were obtained.