Pope C G, Urban B W, Haydon D A
Biochim Biophys Acta. 1982 May 21;688(1):279-83. doi: 10.1016/0005-2736(82)90605-8.
The mean lifetime of gramicidin A channels in bilayers formed from monoolein and squalane was sharply reduced by the absorption of a range of n-alkanols and cholesterol. Results are shown for n-hexanol, n-octanol, n-decanol, n-dodecanol, n-tetradecanol, n-hexadecanol, n-octadecanol and cholesterol. The longer chain n-alkanols were apparently more effective than the shorter members and cholesterol was the most effective of the substances examined. The single channel conductance was also affected, though to a much lesser extent than the mean channel lifetime, the n-alkanols producing increases and cholesterol a decrease. It is suggested that membrane fluidity changes are not likely to be primarily responsible for the reductions in channel lifetimes but that the bilayer tension, which is known to be increased by n-octanol, could be significant.
由单油酸甘油酯和角鲨烷形成的双层膜中短杆菌肽A通道的平均寿命，会因一系列正构烷醇和胆固醇的吸附而急剧缩短。给出了正己醇、正辛醇、正癸醇、正十二醇、正十四醇、正十六醇、正十八醇和胆固醇的结果。长链正构烷醇显然比短链的更有效，而胆固醇是所研究物质中最有效的。单通道电导也受到影响，不过程度远小于平均通道寿命，正构烷醇使其增加，而胆固醇使其降低。有人认为，膜流动性变化不太可能是通道寿命缩短的主要原因，而双层膜张力可能起重要作用，已知正辛醇会增加双层膜张力。
