Elliott J R, Needham D, Dilger J P, Brandt O, Haydon D A
Biochim Biophys Acta. 1985 Apr 11;814(2):401-4. doi: 10.1016/0005-2736(85)90462-6.
The effects of n-decanol, n-hexadecanol, n-octyl(oxyethylene)3 alcohol and cholesterol on gramicidin single-channel lifetime in planar lipid bilayers have been determined. The bilayers used were formed from a solution of monoolein in squalene. Measurements have also been made of the above compounds' effects on membrane thickness (as measured by electrical capacity and optical reflectance technique) and surface tension (as derived from bulk interfacial tension and bilayer-lens contact angle measurements). The reduction in single-channel lifetime caused by the n-alkanols may be accounted for quantitatively in terms of the effects of these compounds on bilayer thickness and surface tension. The n-octyl(oxyethylene)3 alcohol caused an increase in single-channel lifetime which is also consistent with the thickness/tension theory. The reduction in channel lifetime caused by cholesterol, however, was much larger than would be predicted from its effects on bilayer thickness and surface tension.
已测定正癸醇、正十六醇、正辛基（氧乙烯）3醇和胆固醇对平面脂质双分子层中短杆菌肽单通道寿命的影响。所用的双分子层由单油酸甘油酯在角鲨烯中的溶液形成。还测量了上述化合物对膜厚度（通过电容和光反射技术测量）和表面张力（从本体界面张力和双分子层-透镜接触角测量得出）的影响。正链烷醇引起的单通道寿命缩短可以根据这些化合物对双分子层厚度和表面张力的影响进行定量解释。正辛基（氧乙烯）3醇导致单通道寿命增加，这也与厚度/张力理论一致。然而，胆固醇引起的通道寿命缩短比根据其对双分子层厚度和表面张力的影响所预测的要大得多。
