Medullary cells of origin of physiologically identified cardiac nerves in the dog.

Individual cardiac nerves from which stimulation elicited cardioinhibition (bradycardia and negative inotropism) were identified in 24 of 38 dogs. Subsequently, 3-25 microliters of 30% horseradish peroxidase (HRP) were injected into an identified cardiac nerve. After a 3-day survival period, the medulla oblongata was processed for HRP histochemistry. Retrograde labeling was observed to be concentrated primarily in the ipsilateral nucleus ambiguus (NA) and in medium-sized neurons located ventral and lateral to the larger neurons of the principal NA cell column. This latter location was so characteristic that it has been designated the ventrolateral nucleus ambiguus (VLNA). Labeled neurons were found at all levels of the NA and VLNA and their distribution was similar irrespective of the cardiac nerve injected. Relatively few labeled neurons were observed in the dorsal motor nucleus of the vagus nerve (DMV) except after injections into the left and right recurrent cardiac nerves and the left cranial vagal nerve. In some dogs labeled cells were present only in and ventrolateral to the NA and not in the DMV, even though stimulation of the injected nerve elicited both bradycardia and negative inotropism. These results demonstrate that ventrolateral regions of the NA represent the major site of cardioinhibitory motor neurons in the dog that they can regulate both rate and force.