Gil-Turnes C, de Araujo F L
Can J Comp Med. 1982 Apr;46(2):165-8.
Sera were produced in rabbits against nine Moraxella bovis strains isolated in Brazil and three in the United States. Antigens were prepared for double immunodiffusion tests by thawing concentrated suspensions of the strains. Sera were tested against homologous and heterologous antigen preparations by the double immunodiffusion method. Sera showing precipitin bands with heterologous antigens were absorbed. Antigenic differences were detected between the strains and a provisional grouping of strains of M. bovis was suggested on the basis of antigenic composition. Differences between isolates from different geographical locations were found and some strains appeared antigenically more complex than others. The relevance of this work to vaccine production was suggested.
用从巴西分离出的9株牛莫拉菌和从美国分离出的3株牛莫拉菌在兔子体内制备血清。通过解冻菌株的浓缩悬浮液来制备用于双向免疫扩散试验的抗原。采用双向免疫扩散法用血清检测同源和异源抗原制剂。对显示与异源抗原有沉淀带的血清进行吸收处理。检测到菌株之间的抗原差异，并根据抗原组成对牛莫拉菌菌株进行了初步分组。发现来自不同地理位置的分离株之间存在差异，一些菌株的抗原性似乎比其他菌株更复杂。还提出了这项工作与疫苗生产的相关性。
