Buchanan T M, Pearce W A
Infect Immun. 1976 May;13(5):1483-9. doi: 10.1128/iai.13.5.1483-1489.1976.
Isolated pure gonococcal pili were found capable of producing direct agglutination of human erythrocytes. Four different strains of gonococci were compared, and hemagglutination was produced by isolated pili or piliated gonococci but not by nonpiliated gonococci of each strain. Pili from each of the four strains were antigenically distinguishable using antisera specific for pili to agglutinate piliated gonococci, form precipitin lines in Ouchterlony immunodiffusion, or inhibit hemagglutination caused by purified pili or piliated gonococci. However, these tests also demonstrate some shared antigenicity among pili. Shared antigens among the four pili types were quantitated at less than or equal to 2.5% by radioimmunoassay. Inhibition of hemagglutination was most marked with antiserum to the homologous pili type. Inhibition of hemagglutination by antiserum to heterologous pili suggested that shared antigens on pili from B and 2686 strains of gonococci are located near the erythrocyte attachment moiety of B strain pili and removed from the attachment moiety of 2686 strain pili. These results suggest that antigenic heterogeneity of pili will prove an important factor in any efforts to use pili as a vaccine for gonorrhea.
已发现分离出的纯淋菌菌毛能够使人红细胞发生直接凝集。对4种不同的淋球菌菌株进行了比较，发现分离出的菌毛或有菌毛的淋球菌可产生血凝反应，而每种菌株的无菌毛淋球菌则不能。使用针对菌毛的抗血清来凝集有菌毛的淋球菌、在双向免疫扩散中形成沉淀线或抑制由纯化菌毛或有菌毛淋球菌引起的血凝反应，以此可区分4种菌株中每种菌株的菌毛抗原性。然而，这些试验也证明了菌毛之间存在一些共同抗原性。通过放射免疫测定法对4种菌毛类型中的共同抗原进行定量，结果显示其含量小于或等于2.5%。同源菌毛类型的抗血清对血凝反应的抑制作用最为明显。异源菌毛抗血清对血凝反应的抑制作用表明，B株和2686株淋球菌菌毛上的共同抗原位于B株菌毛的红细胞附着部分附近，而与2686株菌毛的附着部分相距较远。这些结果表明，菌毛的抗原异质性将成为把菌毛用作淋病疫苗的任何努力中的一个重要因素。
