Lee T H, Brown M J, Nagy L, Causon R, Walport M J, Kay A B
J Allergy Clin Immunol. 1982 Aug;70(2):73-81. doi: 10.1016/0091-6749(82)90232-9.
Concentrations of plasma histamine and serum neutrophil chemotactic factor (NCF) were measured in seven atopic asthmatics who developed exercise-induced asthma (EIA) after a treadmill task. The results were compared with those obtained after inhalation of specific antigen or methacholine. Plasma histamine concentrations were measured with a novel double-isotope radiometric assay, and NCF was identified by its elution in the void volume fractions of Sephadex G-200 and as a single peak of activity at approximately 0.20 molar NaCl after anion exchange chromatography on diethylaminoethyl-Sephacel (pH 7.8). After exercise or antigen challenge, the time courses of appearance of both mediators were virtually identical and accompanied the increase in airways obstruction. There was a statistically significant correlation between the concentrations of histamine or NCF and the magnitude of airflow obstruction after exercise and antigen challenge. This suggested that there may be a direct association between mediator release and EIA or antigen-induced bronchoconstriction. In contrast, there were no significant elevations in circulating histamine and NCF after inhalation of methacholine, at concentrations giving a fall in FEV1 comparable to that induced by exercise or antigen. The prior administration of cromolyn to three asthmatics inhibited both their EIA and the release of histamine and NCF. When four asthmatics were exercised for periods of 1, 3, and 6 min, the release of NCF and fall in peak expiratory flow rate were directly related to the duration of the exercise. The rise of NCF activity in subjects with EIA was fivefold greater than that observed in asthmatics who did not experience airways obstruction when subjected to the same exercise task. These results provide further evidence that mediators of hypersensitivity are released during EIA.
对7名患运动诱发性哮喘（EIA）的特应性哮喘患者在进行跑步机运动任务后，测量其血浆组胺浓度和血清中性粒细胞趋化因子（NCF）浓度。将结果与吸入特异性抗原或乙酰甲胆碱后获得的结果进行比较。血浆组胺浓度采用新型双同位素放射性测定法测量，NCF通过其在Sephadex G - 200的空体积组分中的洗脱以及在二乙氨基乙基 - Sephacel（pH 7.8）上进行阴离子交换色谱后在约0.20摩尔氯化钠处的单一活性峰来鉴定。运动或抗原激发后，两种介质出现的时间进程几乎相同，并伴随着气道阻塞的增加。运动和抗原激发后，组胺或NCF的浓度与气流阻塞的程度之间存在统计学上的显著相关性。这表明介质释放与EIA或抗原诱导的支气管收缩之间可能存在直接关联。相比之下，吸入乙酰甲胆碱后，在给予与运动或抗原诱导的FEV1下降相当的浓度时，循环组胺和NCF没有显著升高。对3名哮喘患者预先给予色甘酸钠可抑制其EIA以及组胺和NCF的释放。当4名哮喘患者分别运动1、3和6分钟时，NCF的释放和呼气峰值流速的下降与运动持续时间直接相关。EIA患者中NCF活性的升高比在相同运动任务下未经历气道阻塞的哮喘患者中观察到的升高大五倍。这些结果进一步证明了在EIA期间超敏反应介质被释放。
