Beitz A J
J Neurosci. 1982 Jul;2(7):829-42. doi: 10.1523/JNEUROSCI.02-07-00829.1982.
The combined horseradish peroxidase retrograde transport-peroxidase-antiperoxidase immunohistochemical procedure was utilized in the present study to ascertain the sites of origin of serotonin and neurotensin projections to the rodent nucleus raphe magnus. The major serotonin inputs to the raphe magnus arise from the B-8 and B-9 groups of Dahlstrom and Fuxe (Dahlstrom, A., and K. Fuxe (1964) Acta Physiol. Scand. Suppl. 232 62: 1-55), the nucleus reticularis paragigantocellularis, and the nucleus reticularis gigantocellularis pars alpha. Neurotensinergic projections to the raphe magnus originate predominantly from the periaqueductal gray, the nucleus solitarius, the dorsal and ventral parabrachial nuclei, and the nucleus cuneiformis. The periaqueductal gray and the nucleus paragigantocellularis were found to provide both a neurotensin and a serotonin projection to this raphe nucleus. The present results indicate that several brain stem nuclei, which have been implicated previously in endogenous analgesia mechanisms, provide serotonergic and neurotensinergic input to the nucleus raphe magnus.
在本研究中，采用了辣根过氧化物酶逆行运输-过氧化物酶-抗过氧化物酶免疫组织化学联合方法，以确定5-羟色胺和神经降压素投射至啮齿动物中缝大核的起源部位。中缝大核的主要5-羟色胺输入源自达尔斯特伦和富克斯的B-8和B-9组（达尔斯特伦，A.，和K.富克斯（1964年）《生理学杂志》增刊232 62: 1-55）、旁巨细胞网状核以及巨细胞网状核α部。至中缝大核的神经降压素能投射主要起源于导水管周围灰质、孤束核、臂旁背核和腹核以及楔状核。发现导水管周围灰质和旁巨细胞网状核为此中缝核提供神经降压素和5-羟色胺投射。目前的结果表明，先前已牵涉到内源性镇痛机制的几个脑干核向中缝大核提供5-羟色胺能和神经降压素能输入。
