Doehmer J, Barinaga M, Vale W, Rosenfeld M G, Verma I M, Evans R M
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2268-72. doi: 10.1073/pnas.79.7.2268.
We have introduced the rat growth hormone gene into mouse fibroblasts via a retroviral DNA vector. The ability of the viral DNA to induce foci in the recipient cells was used as a dominant selection marker. Several copies of rat growth hormone DNA were integrated in the mouse cells. The transformed mouse cells expressed rat growth hormone-specific mRNA and secreted mature rat growth hormone. In rat cells, the expression of this gene is regulated by glucocorticoids. We demonstrate that hormone-dependent regulation transfers with the clone and thus appears to be an intrinsic property of the gene or its RNA products.
我们通过逆转录病毒DNA载体将大鼠生长激素基因导入小鼠成纤维细胞。病毒DNA在受体细胞中诱导病灶的能力被用作显性选择标记。大鼠生长激素DNA的几个拷贝整合到了小鼠细胞中。转化后的小鼠细胞表达大鼠生长激素特异性mRNA并分泌成熟的大鼠生长激素。在大鼠细胞中，该基因的表达受糖皮质激素调节。我们证明激素依赖性调节与克隆一起转移，因此似乎是该基因或其RNA产物的固有特性。
