Ju S T, Benacerraf B, Dorf M E
J Exp Med. 1980 Jul 1;152(1):170-82. doi: 10.1084/jem.152.1.170.
We developed an idiotypic radioimmunoassay system that detects shared idiotypic determinants, termed GTGL idiotype, on antibodies bearing distinct antigen-binding specificities in various mouse strains. Either poly-(Glu, Tyr) (GT)- or poly-(Glu, Lys) (GL)-related determinants are able to induce anti-GT and anti-GL (GTGL)-idiotypic antibodies. Strain distribution studies indicate that GTGL-idiotypic antibodies are readily induced and frequently expressed in antisera obtained from 25 different mouse strains immunized either with GT-related or GL-related polymers. The ability to express GTGL-idiotypic antibodies is a dominant trait and is controlled by Igh-linked gene(s). In addition, we demonstrated that in anticopolymer of L-glutamine acid60- L-alanine30-L-tyrosine (GAT) and anticopolymer of L-glutamic acid54-L-lysine35-L-phenylalanine11(GLphi) antisera, both antibodies uniquely specific to GAT or GLphi, respectively, and antibodies bearing dual specificities for GAT and GLphi, expressed GTGL idiotype. The genetic implications of these findings are discussed. X
我们开发了一种独特型放射免疫分析系统，该系统可检测在各种小鼠品系中具有不同抗原结合特异性的抗体上共有的独特型决定簇，称为GTGL独特型。聚（谷氨酸，酪氨酸）（GT）或聚（谷氨酸，赖氨酸）（GL）相关决定簇均能够诱导抗GT和抗GL（GTGL）独特型抗体。品系分布研究表明，在用GT相关或GL相关聚合物免疫的25种不同小鼠品系获得的抗血清中，GTGL独特型抗体很容易被诱导并频繁表达。表达GTGL独特型抗体的能力是一种显性性状，由与Igh连锁的基因控制。此外，我们证明，在L-谷氨酸60-L-丙氨酸30-L-酪氨酸（GAT）的抗共聚物和L-谷氨酸54-L-赖氨酸35-L-苯丙氨酸11（GLphi）的抗血清中，分别对GAT或GLphi具有独特特异性的抗体以及对GAT和GLphi具有双重特异性的抗体均表达GTGL独特型。讨论了这些发现的遗传学意义。X
