单克隆T细胞群体的功能性辅助活性：抗原特异性且受H-2限制的克隆化T细胞为体外针对三硝基苯基-聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）的抗体应答提供辅助。
Functional helper activity of monoclonal T cell populations: antigen-specific and H-2 restricted cloned T cells provide help for in vitro antibody responses to trinitrophenyl-poly(LTyr,Glu)-poly(DLAla)--poly(LLys).
作者信息
Hodes R J, Kimoto M, Hathcock K S, Fathman C G, Singer A
出版信息
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6431-5. doi: 10.1073/pnas.78.10.6431.
Abstract
The ability of long-term cultured and monoclonal T cell populations to provide antigen-specific help was assessed in a system of Ir gene-controlled in vitro antibody responses to soluble antigens. T-cell colonies and monoclonal T-cell lines were generated which proliferated specifically in response to poly(LTyr,Glu)-poly(DLAla)--poly(LLys) [(T,G)-A--L] and were I-A restricted in these proliferative responses. These (T,G)-A--L-specific T-cell populations were evaluated for their ability to help unprimed and T-cell depleted spleen cell populations in the generation of antibody responses to trinitrophenyl (TNP)-(T,G)-A--L in vitro. It was found that long-term T-cell lines, including monoclonal T-cell populations derived by limiting dilution, were highly efficient helper cells for IgM responses to TNP-(T,G)-A--L. These helper T cells were both antigen-specific and I-A restricted in their ability to be activated and to cooperate with T-cell depleted spleen cell populations. Once specifically activated, however, these clones provided help that was antigen nonspecific. These studies have thus demonstrated the ability of antigen-specific and H-2-restricted monoclonal T-cell populations to provide help for responses to soluble antigens in vitro.
摘要
在一个受Ir基因控制的针对可溶性抗原的体外抗体反应系统中，评估了长期培养的单克隆T细胞群体提供抗原特异性辅助的能力。生成了T细胞集落和单克隆T细胞系，它们能针对聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）[（T，G）-A-L]特异性增殖，且这些增殖反应受I-A限制。评估了这些针对（T，G）-A-L的特异性T细胞群体在体外对三硝基苯（TNP）-（T，G）-A-L产生抗体反应时，帮助未致敏和T细胞耗竭的脾细胞群体的能力。发现长期T细胞系，包括通过有限稀释获得的单克隆T细胞群体，是对TNP-（T，G）-A-L产生IgM反应的高效辅助细胞。这些辅助性T细胞在被激活以及与T细胞耗竭的脾细胞群体合作的能力方面，既是抗原特异性的又是受I-A限制的。然而，一旦被特异性激活，这些克隆提供的辅助是抗原非特异性的。因此，这些研究证明了抗原特异性和H-2限制的单克隆T细胞群体在体外为针对可溶性抗原的反应提供辅助的能力。
相似文献
1
Functional helper activity of monoclonal T cell populations: antigen-specific and H-2 restricted cloned T cells provide help for in vitro antibody responses to trinitrophenyl-poly(LTyr,Glu)-poly(DLAla)--poly(LLys).单克隆T细胞群体的功能性辅助活性：抗原特异性且受H-2限制的克隆化T细胞为体外针对三硝基苯基-聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）的抗体应答提供辅助。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6431-5. doi: 10.1073/pnas.78.10.6431.
2
3
Elicitation and detection of in vitro H-2-linked Ir gene regulated antibody responses to poly(LTyr, Glu)-poly(DLAla)--poly(LLys).体外诱导和检测与H-2连锁的Ir基因调控的针对聚（L-酪氨酸，谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）的抗体反应
J Immunol Methods. 1983 Mar 11;58(1-2):183-91. doi: 10.1016/0022-1759(83)90274-0.
4
Genetic regulation of delayed-type hypersensitivity responses to poly (Tyr,Glu)-poly(DLAla)--poly(Lys): expression of the genetic defect in the induction and manifestation phases in H-2s and H-2f mice.对聚（酪氨酸，谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）迟发型超敏反应的遗传调控：H-2s和H-2f小鼠诱导期和表现期遗传缺陷的表达
Immunology. 1982 Mar;45(3):405-11.
5
Genetic regulation of delayed-type hypersensitivity responses to poly(LTyr,LGlu)-poly(DLAla)--poly(LLys). II. Evidence for a T-T-cell collaboration in delayed-type hypersensitivity responses and for a T-cell defect at the efferent phase in nonresponder H-2k mice.对聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）--聚（L-赖氨酸）迟发型超敏反应的遗传调控。II. 非应答性H-2k小鼠迟发型超敏反应中T细胞与T细胞协作的证据及传出相T细胞缺陷的证据
J Exp Med. 1980 Mar 1;151(3):628-36. doi: 10.1084/jem.151.3.628.
6
Genetic regulation of delayed-type hypersensitivity responses to poly(LTyr,LGu)-poly(DLAla)--poly(LLys). I. Expression of the genetic defect at two phases of the immune process.对聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）--聚（L-赖氨酸）迟发型超敏反应的遗传调控。I. 免疫过程两个阶段遗传缺陷的表达
J Exp Med. 1980 Feb 1;151(2):265-74. doi: 10.1084/jem.151.2.265.
7
Functional requirements of (Phe, G)-A--L-specific T-cell clones of (H-2b X H-2k)F1 origin.（H-2b×H-2k）F1 来源的（苯丙氨酸，甘氨酸）-A--L 特异性 T 细胞克隆的功能需求。
Immunogenetics. 1986;24(6):386-90. doi: 10.1007/BF00377957.
8
Liposomes as immunological adjuvants in eliciting antibodies specific to the synthetic polypeptide poly(LTyr, LGlu)-poly(DLAla)--(LLys) with high frequency of site-associated idiotypic determinants.脂质体作为免疫佐剂，可高效引发针对合成多肽聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）-（L-赖氨酸）且具有高频位点相关独特型决定簇的特异性抗体。
Eur J Immunol. 1981 May;11(5):398-404. doi: 10.1002/eji.1830110510.
9
IL 2 restores memory B cell activation by antigen-specific T cell clone variants.白细胞介素2可通过抗原特异性T细胞克隆变体恢复记忆B细胞的激活。
J Immunol. 1986 Jan;136(1):39-43.
10
Role of antigenic structure in cell to cell cooperation.抗原结构在细胞间协作中的作用。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4184-6. doi: 10.1073/pnas.73.11.4184.
引用本文的文献
1
Requirement for three signals in B cell responses. II. Analysis of antigen- and Ia-restricted T helper cell-B cell interaction.B细胞应答中三种信号的需求。II. 对抗原及Ia限制的辅助性T细胞与B细胞相互作用的分析。
J Exp Med. 1982 Aug 1;156(2):415-29. doi: 10.1084/jem.156.2.415.
2
Role of the major histocompatibility complex in T cell activation of B cell subpopulations. A single monoclonal T helper cell population activates different B cell subpopulations by distinct pathways.主要组织相容性复合体在B细胞亚群T细胞活化中的作用。单一的单克隆辅助性T细胞群体通过不同途径激活不同的B细胞亚群。
J Exp Med. 1982 Aug 1;156(2):350-60. doi: 10.1084/jem.156.2.350.
3
Single-cell studies on hapten-specific B cells: response to T-cell-dependent antigens.对半抗原特异性B细胞的单细胞研究：对T细胞依赖性抗原的反应
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2479-83. doi: 10.1073/pnas.81.8.2479.
4
Major histocompatibility complex-restricted self-recognition in responses to trinitrophenyl-Ficoll. A novel cell interaction pathway requiring self-recognition of accessory cell H-2 determinants by both T cells and B cells.对三硝基苯基-聚蔗糖反应中的主要组织相容性复合体限制的自身识别。一种新的细胞相互作用途径，需要T细胞和B细胞对辅助细胞H-2决定簇进行自身识别。
J Exp Med. 1983 Feb 1;157(2):486-501. doi: 10.1084/jem.157.2.486.
5
Multiple thymus-derived helper cell activities involved in antibody responses.参与抗体应答的多种胸腺来源辅助性细胞活性。
Surv Immunol Res. 1982;1(2):117-21. doi: 10.1007/BF02918334.
6
T-cell proliferative response to hapten-modified self-immunoglobulins: recognition of conjugate-specific determinants.T细胞对半抗原修饰的自身免疫球蛋白的增殖反应：共轭特异性决定簇的识别
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4156-60. doi: 10.1073/pnas.79.13.4156.
7
T cell regulation of B cell activation. T cells independently regulate the responses mediated by distinct B cell subpopulations.T细胞对B细胞活化的调节。T细胞独立调节由不同B细胞亚群介导的反应。
J Exp Med. 1982 May 1;155(5):1267-76. doi: 10.1084/jem.155.5.1267.
本文引用的文献
1
Role of the major histocompatibility complex in T cell activation of B cell subpopulations Lyb-5+ and Lyb-5- B cell subpopulations differ in their requirement for major histocompatibility complex-restricted T cell recognition.主要组织相容性复合体在B细胞亚群Lyb-5+和Lyb-5-的T细胞激活中的作用Lyb-5+和Lyb-5- B细胞亚群在对主要组织相容性复合体限制的T细胞识别的需求上存在差异。
J Exp Med. 1981 Aug 1;154(2):501-16. doi: 10.1084/jem.154.2.501.
2
H-2-unrestricted polyclonal maturation without replication of small B cells induced by antigen-activated T cell help factors.由抗原激活的T细胞辅助因子诱导的小B细胞的H-2非限制性多克隆成熟且无复制。
Eur J Immunol. 1980 Sep;10(9):679-85. doi: 10.1002/eji.1830100905.
3
Cell cooperation during in vivo anti-hapten antibody responses. V. Two synergistic Ly-1+23- helper T cells with distinctive specificities.体内抗半抗原抗体应答过程中的细胞协作。V. 两种具有独特特异性的协同性Ly-1⁺23⁻辅助性T细胞。
Eur J Immunol. 1980 Apr;10(4):231-6. doi: 10.1002/eji.1830100402.
4
Studies Utilizing murine T cell clones: Ir genes, Ia antigens and MLR stimulating determinants.利用小鼠T细胞克隆的研究：Ir基因、Ia抗原和混合淋巴细胞反应刺激决定簇。
Immunol Rev. 1981;54:57-79. doi: 10.1111/j.1600-065x.1981.tb00434.x.
5
Antigen-reactive T cell clones. II. Unique homozygous and (high responder x low responder)F1 hybrid antigen-presenting determinants detected using poly(Tyr, Glu)-poly D, L-Ala--poly Lys-reactive T cell clones.抗原反应性T细胞克隆。II. 使用聚（酪氨酸，谷氨酸）-聚D，L-丙氨酸-聚赖氨酸反应性T细胞克隆检测到的独特纯合子和（高反应者×低反应者）F1杂种抗原呈递决定簇。
J Exp Med. 1981 Feb 1;153(2):375-85. doi: 10.1084/jem.153.2.375.
6
Antigen-specific helper T cells required for dominant production of an idiotype (THId) are not under immune response (Ir) gene control.独特型（THId）优势产生所需的抗原特异性辅助性T细胞不受免疫反应（Ir）基因控制。
J Exp Med. 1980 Dec 1;152(6):1571-82. doi: 10.1084/jem.152.6.1571.
7
Direct cloning and extended culture of antigen-specific MHC-restricted, proliferating T lymphocytes.抗原特异性MHC限制的增殖性T淋巴细胞的直接克隆与长期培养。
Nature. 1980 Feb 7;283(5747):581-3. doi: 10.1038/283581a0.
8
Cell-bound receptors for alloantigens on normal lymphocytes. II. Antialloantibody serum contains specific factors reacting with relevant immunocompetent T lymphocytes.正常淋巴细胞上同种异体抗原的细胞结合受体。II. 抗同种异体抗体血清含有与相关免疫活性T淋巴细胞发生反应的特异性因子。
J Exp Med. 1974 Sep 1;140(3):731-41. doi: 10.1084/jem.140.3.731.
9
Cell interactions between histoincompatible T and B lymphocytes. II. Failure of physiologic cooperative interactions between T and B lymphocytes from allogeneic donor strains in humoral response to hapten-protein conjugates.组织相容性不相容的T淋巴细胞和B淋巴细胞之间的细胞相互作用。II. 来自同种异体供体品系的T淋巴细胞和B淋巴细胞在对半抗原-蛋白质结合物的体液反应中缺乏生理性协同相互作用。
J Exp Med. 1973 Jun 1;137(6):1405-18. doi: 10.1084/jem.137.6.1405.
10
The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens.巨噬细胞在辅助性T细胞生成中的作用。II. 巨噬细胞与T细胞相互作用以通过可溶性抗原诱导辅助性细胞的遗传控制。
J Exp Med. 1975 Aug 1;142(2):460-72. doi: 10.1084/jem.142.2.460.
Zotero 插件