Claas F H, Runia-van Nieuwkoop R, van den Berge W, van Rood J J
Hum Immunol. 1982 Aug;5(1):83-90. doi: 10.1016/0198-8859(82)90033-7.
When lymphocytes of healthy donors were incubated with penicillin and washed before HLA typing, the cytotoxic reactivity of some typing sera with their corresponding antigen was blocked. When the penicillin was added to the cytotoxicity assay at different times, competition between penicillin and alloantiserum for binding on or near the same epitope on the HLA molecule could be observed. On the basis of the inhibition experiments, HLA antigens can be divided into three groups: 1. Antibody reactivity blocked by penicillin (Bw35) 2. Antibody reactivity not blocked by penicillin (B14) 3. Antibody reactivity blocked by penicillin in some donors but not in others (A2). This third group may provide an opportunity to determine structural differences among, until now, serologically indistinguishable HLA molecules. By adding penicillin to a multispecific serum, containing both antibodies that can and cannot be blocked, a monospecific typing serum can be obtained. The differential effect of penicillin on the HLA antigens was not restricted to complement-dependent cytotoxicity on lymphocytes. Similar results were obtained when the HLA antisera were tested with indirect immunofluorescence on platelets or in absorption experiments.
当健康供体的淋巴细胞与青霉素一起孵育并在进行HLA分型前洗涤时，一些分型血清与其相应抗原的细胞毒性反应性被阻断。当在不同时间将青霉素加入细胞毒性试验中时，可以观察到青霉素和同种抗血清在HLA分子上或其附近的相同表位上结合的竞争。基于抑制实验，HLA抗原可分为三组：1. 抗体反应性被青霉素阻断（Bw35）；2. 抗体反应性不被青霉素阻断（B14）；3. 抗体反应性在一些供体中被青霉素阻断而在另一些供体中不被阻断（A2）。这第三组可能为确定迄今为止血清学上无法区分的HLA分子之间的结构差异提供机会。通过向含有可被阻断和不可被阻断抗体的多特异性血清中加入青霉素，可以获得单特异性分型血清。青霉素对HLA抗原的差异作用不仅限于淋巴细胞上的补体依赖性细胞毒性。当用血小板进行间接免疫荧光试验或在吸收实验中检测HLA抗血清时，也得到了类似的结果。
