B族链球菌抗体酶联免疫吸附测定的免疫特异性及定量分析
Immunospecificity and quantitation of an enzyme-linked immunosorbent assay for group B streptococcal antibody.
作者信息
Anthony B F, Concepcion N F, McGeary S A, Ward J I, Heiner D C, Shapshak P, Insel R A
出版信息
J Clin Microbiol. 1982 Aug;16(2):350-4. doi: 10.1128/jcm.16.2.350-354.1982.
Abstract
Type-specific antigen was purified from the supernatant of type III group B streptococcal cultures, tyrosylated, and bound to microtiter wells for an enzyme-linked immunosorbent assay. The immunological specificity of the antigen and the assay was shown by (i) reaction only with homologous unabsorbed rabbit sera and (ii) inhibition after incubation of human serum with homologous but not heterologous purified antigen. The assay was quantitated by relating optical density readings to absolute amounts of human immunoglobulin G bound to the microtiter wells.
摘要
从B族链球菌III型培养物的上清液中纯化型特异性抗原，进行酪氨酸化处理，然后结合到微量滴定板孔中用于酶联免疫吸附测定。该抗原和测定方法的免疫特异性表现为：（i）仅与同源未吸收兔血清发生反应；（ii）人血清与同源而非异源纯化抗原孵育后出现抑制现象。通过将光密度读数与结合到微量滴定板孔中的人免疫球蛋白G的绝对量相关联来对该测定进行定量。
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