用于干扰素的自动化定量细胞病变效应降低试验
Automated, quantitative cytopathic effect reduction assay for interferon.
作者信息
Yeh T J, McBride P T, Overall J C, Green J A
出版信息
J Clin Microbiol. 1982 Aug;16(2):413-5. doi: 10.1128/jcm.16.2.413-415.1982.
Abstract
A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.
摘要
本文描述了一种用于检测人干扰素（IFN）的快速细胞病变效应降低试验。使用自动稀释仪在96孔微量滴定板中对IFN进行稀释。总孵育时间为26小时。通过自动分光光度计读取结晶紫染色单层的光密度读数来计算IFN效价，读取每块板所需时间不到1分钟。
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本文引用的文献
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2
Convenient assay for interferons.干扰素的便捷检测方法。
J Virol. 1981 Feb;37(2):755-8. doi: 10.1128/JVI.37.2.755-758.1981.
3
Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons.病毒诱导的和免疫的人干扰素的快速、定量、半自动检测法。
J Clin Microbiol. 1980 Sep;12(3):433-8. doi: 10.1128/jcm.12.3.433-438.1980.
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Microassay for human and chick cell interferons.人和鸡细胞干扰素的微量测定法。
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Rapid assay of interferon.干扰素快速检测
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Potentiation of interferon activity by mixed preparations of fibroblast and immune interferon.成纤维细胞干扰素与免疫干扰素混合制剂对干扰素活性的增强作用。
Infect Immun. 1979 Oct;26(1):248-53. doi: 10.1128/iai.26.1.248-253.1979.
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Appl Environ Microbiol. 1976 Jan;31(1):35-8. doi: 10.1128/aem.31.1.35-38.1976.
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