用于干扰素的自动化定量细胞病变效应降低试验
Automated, quantitative cytopathic effect reduction assay for interferon.
作者信息
Yeh T J, McBride P T, Overall J C, Green J A
出版信息
J Clin Microbiol. 1982 Aug;16(2):413-5. doi: 10.1128/jcm.16.2.413-415.1982.
Abstract
A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.
摘要
本文描述了一种用于检测人干扰素（IFN）的快速细胞病变效应降低试验。使用自动稀释仪在96孔微量滴定板中对IFN进行稀释。总孵育时间为26小时。通过自动分光光度计读取结晶紫染色单层的光密度读数来计算IFN效价，读取每块板所需时间不到1分钟。
相似文献
1
Automated, quantitative cytopathic effect reduction assay for interferon.用于干扰素的自动化定量细胞病变效应降低试验
J Clin Microbiol. 1982 Aug;16(2):413-5. doi: 10.1128/jcm.16.2.413-415.1982.
2
Rapid bioassay of human interferon by direct enzyme immunoassay of encephalomyocarditis virus in HEp-2 cell monolayers after a single cycle of infection.在单轮感染后，通过对人胚肾293（HEp-2）细胞单层中的脑心肌炎病毒进行直接酶免疫测定来快速生物测定人干扰素。
J Virol Methods. 1989 Apr-May;24(1-2):153-8. doi: 10.1016/0166-0934(89)90017-7.
3
A new assay system for guinea pig interferon biological activity.
J Interferon Cytokine Res. 2002 Jul;22(7):793-7. doi: 10.1089/107999002320271387.
4
Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.干扰素的微量滴定测定法：细胞病变效应的显微分光光度法定量
Appl Environ Microbiol. 1976 Jan;31(1):35-8. doi: 10.1128/aem.31.1.35-38.1976.
5
Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons.病毒诱导的和免疫的人干扰素的快速、定量、半自动检测法。
J Clin Microbiol. 1980 Sep;12(3):433-8. doi: 10.1128/jcm.12.3.433-438.1980.
6
New simple dye-uptake assay for interferon.一种新的干扰素简单染料摄取测定法。
Biken J. 1981 Sep;24(3):103-8.
7
A practical cytopathic effect/dye-uptake interferon assay for routine use in the clinical laboratory.
Am J Clin Pathol. 1985 Jun;83(6):735-40. doi: 10.1093/ajcp/83.6.735.
8
Interferon bioassay methods and the development of standard procedures: a critique and analysis of current observations.
In Vitro Monogr. 1974(3):26-34.
9
Synergism between recombinant human interferon and nucleoside antiviral agents against herpes simplex virus: examination with an automated microtiter plate assay.重组人干扰素与核苷类抗病毒药物对单纯疱疹病毒的协同作用：采用自动微量滴定板法检测
J Infect Dis. 1985 Jun;151(6):1116-22. doi: 10.1093/infdis/151.6.1116.
10
Convenient assay for interferons.干扰素的便捷检测方法。
J Virol. 1981 Feb;37(2):755-8. doi: 10.1128/JVI.37.2.755-758.1981.
引用本文的文献
1
Naturally Occurring Fc-Dependent Antibody From HIV-Seronegative Individuals Promotes HIV-Induced IFN-α Production.来自 HIV 血清阴性个体的天然存在的 Fc 依赖性抗体促进 HIV 诱导的 IFN-α 产生。
Sci Rep. 2016 Nov 24;6:37493. doi: 10.1038/srep37493.
2
Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β.建立一个能够高水平表达重组猪 IFN-β的稳定 CHO 细胞系。
Cytokine. 2011 Jun;54(3):324-9. doi: 10.1016/j.cyto.2010.12.002. Epub 2011 Apr 1.
3
Identification of alpha interferon-induced genes associated with antiviral activity in Daudi cells and characterization of IFIT3 as a novel antiviral gene.鉴定与 Daudi 细胞抗病毒活性相关的α干扰素诱导基因，并鉴定 IFIT3 为一种新的抗病毒基因。
J Virol. 2010 Oct;84(20):10671-80. doi: 10.1128/JVI.00818-10. Epub 2010 Aug 4.
4
B and CD4+ T-cell expression of TLR2 is critical for optimal induction of a T-cell-dependent humoral immune response to intact Streptococcus pneumoniae.Toll样受体2（TLR2）在B细胞和CD4 + T细胞中的表达对于诱导针对完整肺炎链球菌的T细胞依赖性体液免疫反应达到最佳状态至关重要。
Eur J Immunol. 2008 Dec;38(12):3316-26. doi: 10.1002/eji.200838484.
5
Two interferons alpha influence each other during their interaction with the extracellular domain of human type interferon receptor subunit 2.两种α干扰素在与人干扰素受体亚基2的细胞外结构域相互作用过程中相互影响。
Biochemistry. 2007 Dec 18;46(50):14638-49. doi: 10.1021/bi7012036. Epub 2007 Nov 21.
6
Evasion of host defenses by measles virus: wild-type measles virus infection interferes with induction of Alpha/Beta interferon production.麻疹病毒对宿主防御的逃避：野生型麻疹病毒感染会干扰α/β干扰素的产生诱导。
J Virol. 2000 Aug;74(16):7478-84. doi: 10.1128/jvi.74.16.7478-7484.2000.
7
Cystamine potently suppresses in vitro HIV replication in acutely and chronically infected human cells.
J Clin Invest. 1994 May;93(5):2251-7. doi: 10.1172/JCI117223.
8
Influenza vaccination and theophylline pharmacokinetics in patients with chronic obstructive lung disease.慢性阻塞性肺疾病患者的流感疫苗接种与茶碱药代动力学
West J Med. 1983 Nov;139(5):651-4.
9
Human polymorphonuclear neutrophil functions are unaffected by human interferon-alpha 2.人类多形核中性粒细胞的功能不受人干扰素-α2的影响。
Infect Immun. 1983 Dec;42(3):1195-7. doi: 10.1128/iai.42.3.1195-1197.1983.
10
Intranasal interferon-alpha 2 for prevention of natural rhinovirus colds.鼻内注射α-2干扰素预防自然感染鼻病毒引起的感冒。
Antimicrob Agents Chemother. 1984 Jul;26(1):31-4. doi: 10.1128/AAC.26.1.31.
本文引用的文献
1
2
Convenient assay for interferons.干扰素的便捷检测方法。
J Virol. 1981 Feb;37(2):755-8. doi: 10.1128/JVI.37.2.755-758.1981.
3
Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons.病毒诱导的和免疫的人干扰素的快速、定量、半自动检测法。
J Clin Microbiol. 1980 Sep;12(3):433-8. doi: 10.1128/jcm.12.3.433-438.1980.
4
Microassay for human and chick cell interferons.人和鸡细胞干扰素的微量测定法。
Appl Microbiol. 1968 Nov;16(11):1706-7. doi: 10.1128/am.16.11.1706-1707.1968.
5
Semi-micro, dye-binding assay for rabbit interferon.兔干扰素的半微量染料结合测定法。
Appl Microbiol. 1971 Apr;21(4):723-5. doi: 10.1128/am.21.4.723-725.1971.
6
Rapid assay of interferon.干扰素快速检测
Tex Rep Biol Med. 1977;35:167-72.
7
Potentiation of interferon activity by mixed preparations of fibroblast and immune interferon.成纤维细胞干扰素与免疫干扰素混合制剂对干扰素活性的增强作用。
Infect Immun. 1979 Oct;26(1):248-53. doi: 10.1128/iai.26.1.248-253.1979.
8
Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.干扰素的微量滴定测定法：细胞病变效应的显微分光光度法定量
Appl Environ Microbiol. 1976 Jan;31(1):35-8. doi: 10.1128/aem.31.1.35-38.1976.
Zotero 插件