恒河猴过敏性神经炎的诱发
Induction of allergic neuritis in rhesus monkeys.
作者信息
Eylar E H, Toro-Goyco E, Kessler M J, Szymanska I
出版信息
J Neuroimmunol. 1982 Oct;3(2):91-8. doi: 10.1016/0165-5728(82)90043-1.
Experimental allergic neuritis (EAN) was induced in rhesus monkey (Macaca mulatta) following sensitization with rabbit nerve (PNS) myelin in complete Freund's adjuvant (CFA) or with bovine P2 protein complexed with phosphatidyl serine (P2-lipid) in CFA. The response of monkeys receiving PNS myelin in CFA differed from the previous studies where monkeys developed clinical signs of fatal EAN within 15-20 days following sensitization. The monkeys in this study (6) showed a much longer delay (40-114 days) before the appearance of severe clinical signs, and 4 of the 6 animals survived without further attack (1 year). Monkeys (4) injected with P2-lipid (2:1 ratio; w/w) developed severe clinical signs of EAN which was fetal in 3 cases. Peripheral lymphocytes from monkeys sensitized to the P2-lipid showed a much stronger mitogeneic response to P2 protein than those from the PNS myelin-sensitized monkeys. on quantitation of the circulating anti-P2 antibodies, the P2-sensitized monkeys generally had much titers than those sensitized with PNS myelin.
用兔神经（周围神经系统，PNS）髓磷脂与完全弗氏佐剂（CFA）致敏，或用与磷脂酰丝氨酸复合的牛P2蛋白（P2-脂质）与CFA致敏后，在恒河猴（猕猴）中诱发实验性变应性神经炎（EAN）。接受CFA中PNS髓磷脂的猴子的反应与之前的研究不同，之前的研究中猴子在致敏后15 - 20天内出现致命性EAN的临床症状。本研究中的猴子（6只）在出现严重临床症状之前有更长的延迟（40 - 114天），6只动物中有4只存活且未再次发作（1年）。注射P2-脂质（2:1比例；重量/重量）的猴子（4只）出现了EAN的严重临床症状，其中3例死亡。对P2-脂质致敏的猴子的外周淋巴细胞对P2蛋白的促有丝分裂反应比对PNS髓磷脂致敏的猴子的外周淋巴细胞强得多。在定量循环抗P2抗体时，P2致敏的猴子的抗体效价通常比用PNS髓磷脂致敏的猴子高得多。
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