Reinecke M, Weihe E, Carraway R E, Leeman S E, Forssmann W G
Neuroscience. 1982 Jul;7(7):1785-95. doi: 10.1016/0306-4522(82)90036-7.
By the use of the peroxidase-antiperoxidase technique guinea-pig hearts were investigated for the occurrence of neurotensin immunoreactivity. Neurotensin immunoreactive nerve fibers were found in distinct localisations in all hearts studied. In addition, neurotensin immunoreactive fibers were present in the adventitia of the ascending aorta, the aortic arch and the pulmonary trunk. All segments of the coronary vasculature exhibited a dense network of neurotensin immunoreactive fibers. This innervation pattern was most pronounced in the arterial portions. Neurotensin immunoreactive fibers occurred also in close contact with atrial and ventricular muscle cells. A particularly dense innervation by neurotensin immunoreactive fibers was present in the sinu-atrial node and in the atrio-ventricular node. The fibers were associated intimately with blood vessels as well as with nodal cells. In addition, neurotensin immunoreactive fibers were found in intracardiac ganglia. The presence of neurotensin-like immunoreactive material in the guinea-pig heart was demonstrated also by radioimmunoassay. The results of immunohistochemistry and radioimmunoassay were correlated. High performance liquid chromatographic analysis indicated that 20-30% of the total immunoreactivity co-chromatographed with guinea-pig or synthetic neurotensin. Evaluation of consecutive sections revealed different innervation patterns of neurotensin and substance P immunoreactive fibers. The findings suggest a neurotransmitter and/or neuromodulator function of neurotensin in the regulation of coronary circulation, of cardiac impulse generation and conduction, of heart muscle contractility and of cardiac reflex mechanisms. It is speculated that neurotensin might represent the efferent and substance P the afferent part of a cardiac regulatory system.
运用过氧化物酶-抗过氧化物酶技术，对豚鼠心脏进行神经降压素免疫反应性的研究。在所研究的所有心脏中，均在不同部位发现了神经降压素免疫反应性神经纤维。此外，在升主动脉、主动脉弓和肺动脉干的外膜中也存在神经降压素免疫反应性纤维。冠状动脉血管的所有节段均呈现出密集的神经降压素免疫反应性纤维网络。这种神经支配模式在动脉部分最为明显。神经降压素免疫反应性纤维也与心房和心室肌细胞紧密接触。在窦房结和房室结中，神经降压素免疫反应性纤维的支配尤为密集。这些纤维与血管以及结细胞紧密相连。此外，在心内神经节中也发现了神经降压素免疫反应性纤维。放射免疫测定法也证实了豚鼠心脏中存在神经降压素样免疫反应性物质。免疫组织化学和放射免疫测定的结果相互关联。高效液相色谱分析表明，总免疫反应性的20% - 30%与豚鼠或合成神经降压素共色谱。对连续切片的评估揭示了神经降压素和P物质免疫反应性纤维的不同神经支配模式。这些发现提示神经降压素在冠状动脉循环调节、心脏冲动产生与传导、心肌收缩力以及心脏反射机制中具有神经递质和/或神经调质功能。据推测，神经降压素可能代表心脏调节系统的传出部分，而P物质代表传入部分。
