化学合成的人α1干扰素基因的表达
Expression of a chemically synthesized human alpha 1 interferon gene.
作者信息
De Maeyer E, Skup D, Prasad K S, De Maeyer-Guignard J, Williams B, Meacock P, Sharpe G, Pioli D, Hennam J, Schuch W, Atherton K
出版信息
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4256-9. doi: 10.1073/pnas.79.14.4256.
Abstract
Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.
摘要
含有化学合成的人α1干扰素（IFN-α1）基因的大肠杆菌细胞，在乳糖启动子的控制下，产生的产物具有与天然IFN-α1无法区分的生物学特性[抗病毒活性、酸稳定性、种间交叉反应性、被针对白细胞或Namalwa细胞干扰素的抗血清灭活，以及刺激（2'-5'）寡腺苷酸合成酶活性]。当将表达单元（乳糖启动子加合成的IFN-α1基因）移植到专性甲基营养菌嗜甲基甲基ophilus甲基otrophus中时，获得了相似水平的干扰素合成。
相似文献
1
Expression of a chemically synthesized human alpha 1 interferon gene.化学合成的人α1干扰素基因的表达
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4256-9. doi: 10.1073/pnas.79.14.4256.
2
3
Comparison of pharmacokinetic behaviors of two human interferons (Lb-IFN-alpha and Re-IFN-alpha A) in cynomolgus monkeys by 2'-5' oligoadenylate synthetase assay.
Jpn J Med Sci Biol. 1986 Oct-Dec;39(5-6):185-98. doi: 10.7883/yoken1952.39.185.
4
A bioassay for serum interferon based on induction of 2'5'-oligoadenylate synthetase activity.
J Interferon Cytokine Res. 1998 Dec;18(12):1011-8. doi: 10.1089/jir.1998.18.1011.
5
Total synthesis of a human leukocyte interferon gene.人白细胞干扰素基因的全合成
Nature. 1981 Aug 20;292(5825):756-62. doi: 10.1038/292756a0.
6
Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.人α-2干扰素基因的化学合成及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Sep 24;11(18):6419-35. doi: 10.1093/nar/11.18.6419.
7
Antiviral activities of hybrids of two major human leukocyte interferons.两种主要人类白细胞干扰素杂交体的抗病毒活性
Nucleic Acids Res. 1981 Nov 25;9(22):6153-66. doi: 10.1093/nar/9.22.6153.
8
IFN-alpha5 mediates stronger Tyk2-stat-dependent activation and higher expression of 2',5'-oligoadenylate synthetase than IFN-alpha2 in liver cells.
J Interferon Cytokine Res. 2004 Aug;24(8):497-503. doi: 10.1089/1079990041689601.
9
Expression of human fibroblast interferon gene in Escherichia coli.人成纤维细胞干扰素基因在大肠杆菌中的表达。
Nature. 1980 Sep 18;287(5779):193-7. doi: 10.1038/287193a0.
10
Identification by electron microscopy of the maturation steps in vaccinia virus morphogenesis inhibited by the interferon-induced enzymes, protein kinase (PKR), 2-5A synthetase, and nitric oxide synthase (iNOS).通过电子显微镜鉴定痘苗病毒形态发生过程中受干扰素诱导酶、蛋白激酶（PKR）、2-5A合成酶和一氧化氮合酶（iNOS）抑制的成熟步骤。
J Interferon Cytokine Res. 2000 Oct;20(10):867-77. doi: 10.1089/10799900050163235.
引用本文的文献
1
Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei.实现低成本生物制药：里氏木霉中高水平干扰素α-2b的生产。
Microb Cell Fact. 2016 Jun 10;15(1):104. doi: 10.1186/s12934-016-0508-5.
2
Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b.重组人干扰素α2b的异源表达、免疫化学及计算分析
Springerplus. 2013 Jun 15;2(1):264. doi: 10.1186/2193-1801-2-264. Print 2013 Dec.
3
Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation.人睾丸间质细胞对腮腺炎病毒感染或聚肌胞刺激的抗病毒反应。
Hum Reprod. 2008 Sep;23(9):2095-103. doi: 10.1093/humrep/den207. Epub 2008 Jun 20.
4
High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.使用原核表达载体对化学合成的人γ干扰素基因进行高水平表达。
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2290-4. doi: 10.1073/pnas.81.8.2290.
5
Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.人α-2干扰素基因的化学合成及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Sep 24;11(18):6419-35. doi: 10.1093/nar/11.18.6419.
6
Interferon-induced 2-5A synthetase activity in human peripheral blood mononuclear cells after immunization with influenza virus and rubella virus vaccines.流感病毒和风疹病毒疫苗免疫后人体外周血单个核细胞中干扰素诱导的2-5A合成酶活性
J Virol. 1984 Mar;49(3):748-53. doi: 10.1128/JVI.49.3.748-753.1984.
7
The purification of eukaryotic polypeptides synthesized in Escherichia coli.在大肠杆菌中合成的真核生物多肽的纯化。
Biochem J. 1986 Nov 15;240(1):1-12. doi: 10.1042/bj2400001.
8
An embryonic DNA-binding protein specific for a region of the human IFN beta 1 promoter.一种对人干扰素β1启动子区域具有特异性的胚胎DNA结合蛋白。
Nucleic Acids Res. 1988 Nov 25;16(22):10575-92. doi: 10.1093/nar/16.22.10575.
9
Role of the SulB (FtsZ) protein in division inhibition during the SOS response in Escherichia coli: FtsZ stabilizes the inhibitor SulA in maxicells.SulB（FtsZ）蛋白在大肠杆菌SOS反应期间的分裂抑制中的作用：FtsZ在极大细胞中稳定抑制剂SulA。
Proc Natl Acad Sci U S A. 1985 Sep;82(18):6045-9. doi: 10.1073/pnas.82.18.6045.
10
Human 2-5A synthetase: characterization of a novel cDNA and corresponding gene structure.人2-5A合成酶：一种新型cDNA及相应基因结构的表征
EMBO J. 1985 Jul;4(7):1761-8. doi: 10.1002/j.1460-2075.1985.tb03848.x.
本文引用的文献
1
Virus interference. II. Some properties of interferon.病毒干扰。二、干扰素的一些特性。
Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):268-73. doi: 10.1098/rspb.1957.0049.
2
Cloning of bovine growth hormone gene and its expression in bacteria.牛生长激素基因的克隆及其在细菌中的表达。
Nucleic Acids Res. 1981 Jan 10;9(1):19-30. doi: 10.1093/nar/9.1.19.
3
Expression of eukaryotic coding sequences in Methylophilus methylotrophus.
Nature. 1982 May 6;297(5861):80-2. doi: 10.1038/297080a0.
4
Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus.嗜甲基甲基ophilus菌对甲醇转化为单细胞蛋白的转化率提高。
Nature. 1980 Oct 2;287(5781):396-401. doi: 10.1038/287396a0.
5
Production in B. subtilis of hepatitis B core antigen and a major antigen of foot and mouth disease virus.枯草芽孢杆菌中乙型肝炎核心抗原和口蹄疫病毒主要抗原的生产。
Nature. 1981 Oct 8;293(5832):481-3. doi: 10.1038/293481a0.
6
Expression of a human gene for interferon in yeast.干扰素人类基因在酵母中的表达。
Nature. 1981 Oct 29;293(5835):717-22. doi: 10.1038/293717a0.
7
Total synthesis of a human leukocyte interferon gene.人白细胞干扰素基因的全合成
Nature. 1981 Aug 20;292(5825):756-62. doi: 10.1038/292756a0.
8
Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them.在大肠杆菌中产生的两种人α干扰素及由它们衍生的杂交分子的靶细胞特异性。
Proc Natl Acad Sci U S A. 1981 May;78(5):2848-52. doi: 10.1073/pnas.78.5.2848.
9
Bacterial synthesis of a novel human leukocyte interferon.一种新型人白细胞干扰素的细菌合成。
Nucleic Acids Res. 1981 Feb 11;9(3):731-41. doi: 10.1093/nar/9.3.731.
10
Comparisons of several biological and physicochemical properties of human leukocyte interferons produced my human leukocytes and by E. coli.对人白细胞产生的人白细胞干扰素和大肠杆菌产生的人白细胞干扰素的几种生物学和物理化学特性进行比较。
Gene. 1980 Nov;11(3-4):181-6. doi: 10.1016/0378-1119(80)90058-x.
Zotero 插件