De Maeyer E, Skup D, Prasad K S, De Maeyer-Guignard J, Williams B, Meacock P, Sharpe G, Pioli D, Hennam J, Schuch W, Atherton K
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4256-9. doi: 10.1073/pnas.79.14.4256.
Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.
含有化学合成的人α1干扰素(IFN-α1)基因的大肠杆菌细胞,在乳糖启动子的控制下,产生的产物具有与天然IFN-α1无法区分的生物学特性[抗病毒活性、酸稳定性、种间交叉反应性、被针对白细胞或Namalwa细胞干扰素的抗血清灭活,以及刺激(2'-5')寡腺苷酸合成酶活性]。当将表达单元(乳糖启动子加合成的IFN-α1基因)移植到专性甲基营养菌嗜甲基甲基ophilus甲基otrophus中时,获得了相似水平的干扰素合成。