一种新型人白细胞干扰素的细菌合成。
Bacterial synthesis of a novel human leukocyte interferon.
作者信息
Yelverton E, Leung D, Weck P, Gray P W, Goeddel D V
出版信息
Nucleic Acids Res. 1981 Feb 11;9(3):731-41. doi: 10.1093/nar/9.3.731.
Abstract
A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. LeIF B exhibits markedly different specificities from another bacterially synthesized human leukocyte interferon, LeIF A.
摘要
在使用成髓细胞样细胞系的聚腺苷酸化信使核糖核酸制备的互补脱氧核糖核酸文库中，鉴定出了一种新型人类白细胞干扰素互补脱氧核糖核酸克隆（LeIF B）。LeIF B的核苷酸序列与其他已发表的白细胞干扰素互补脱氧核糖核酸序列有显著差异。构建了一种表达质粒，该质粒可指导大肠杆菌中每升培养物合成8×10⁷个干扰素单位。LeIF B与另一种细菌合成的人类白细胞干扰素LeIF A表现出明显不同的特异性。
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本文引用的文献
1
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.在大肠杆菌中合成具有人白细胞干扰素活性的多肽。
Nature. 1980 Mar 27;284(5754):316-20. doi: 10.1038/284316a0.
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Synthesis of human fibroblast interferon by E. coli.大肠杆菌合成人成纤维细胞干扰素。
Nucleic Acids Res. 1980 Sep 25;8(18):4057-74. doi: 10.1093/nar/8.18.4057.
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Human leukocyte interferon produced by E. coli is biologically active.由大肠杆菌产生的人白细胞干扰素具有生物活性。
Nature. 1980 Oct 2;287(5781):411-6. doi: 10.1038/287411a0.
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Nature. 1980 Oct 2;287(5781):401-8. doi: 10.1038/287401a0.
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At least three human type alpha interferons: structure of alpha 2.至少三种人α型干扰素：α2的结构
Science. 1980 Sep 19;209(4463):1343-7. doi: 10.1126/science.6158094.
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