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1
Antiviral activities of hybrids of two major human leukocyte interferons.两种主要人类白细胞干扰素杂交体的抗病毒活性
Nucleic Acids Res. 1981 Nov 25;9(22):6153-66. doi: 10.1093/nar/9.22.6153.
2
Carboxyterminal region of hybrid leukocyte interferons affects antiviral specificity.杂交白细胞干扰素的羧基末端区域影响抗病毒特异性。
DNA. 1982;1(3):223-30. doi: 10.1089/dna.1.1982.1.223.
3
Comparison of the antiviral activities of various cloned human interferon-alpha subtypes in mammalian cell cultures.多种克隆的人α干扰素亚型在哺乳动物细胞培养物中的抗病毒活性比较。
J Gen Virol. 1981 Nov;57(Pt 1):233-7. doi: 10.1099/0022-1317-57-1-233.
4
[Comparative study of the biological properties of plasmid and natural human interferons].[质粒干扰素与天然人干扰素生物学特性的比较研究]
Vopr Virusol. 1983 Jan-Feb;28(1):14-21.
5
Antiviral and protein-inducing activities of recombinant human leukocyte interferons and their hybrids.重组人白细胞干扰素及其杂种的抗病毒和诱导蛋白活性
J Virol. 1984 May;50(2):445-50. doi: 10.1128/JVI.50.2.445-450.1984.
6
Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them.在大肠杆菌中产生的两种人α干扰素及由它们衍生的杂交分子的靶细胞特异性。
Proc Natl Acad Sci U S A. 1981 May;78(5):2848-52. doi: 10.1073/pnas.78.5.2848.
7
Human leukocyte interferon produced by E. coli is biologically active.由大肠杆菌产生的人白细胞干扰素具有生物活性。
Nature. 1980 Oct 2;287(5781):411-6. doi: 10.1038/287411a0.
8
Mechanism of interferon action. Kinetics of induction of the antiviral state and protein phosphorylation in mouse fibroblasts treated with natural and cloned interferons.干扰素作用机制。天然干扰素和克隆干扰素处理的小鼠成纤维细胞中抗病毒状态诱导及蛋白质磷酸化的动力学。
J Biol Chem. 1982 Oct 10;257(19):11791-5.
9
Synthesis, antiviral activity, and conformational characterization of mouse-human alpha-interferon hybrids.小鼠-人α-干扰素杂合体的合成、抗病毒活性及构象表征
J Biol Chem. 1988 Jun 25;263(18):8943-52.
10
Specific molecular activities of recombinant and hybrid leukocyte interferons.重组和杂交白细胞干扰素的特定分子活性。
J Biol Chem. 1982 Oct 10;257(19):11497-502.

引用本文的文献

1
Interferon alfacon-1 protects hamsters from lethal pichinde virus infection.干扰素α-1可保护仓鼠免受致死性皮钦德病毒感染。
Antimicrob Agents Chemother. 2005 Jun;49(6):2378-86. doi: 10.1128/AAC.49.6.2378-2386.2005.
2
Signal transduction mediated by growth hormone receptor and its chimeric molecules with the granulocyte colony-stimulating factor receptor.由生长激素受体及其与粒细胞集落刺激因子受体的嵌合分子介导的信号转导。
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):123-7. doi: 10.1073/pnas.90.1.123.
3
High-level expression of an interferon alpha 2 gene cloned in phage M13mp7 and subsequent purification with a monoclonal antibody.克隆于噬菌体M13mp7的干扰素α2基因的高水平表达及随后用单克隆抗体进行的纯化。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5455-9. doi: 10.1073/pnas.79.18.5455.
4
Biological and antigenic similarities of murine interferon-gamma and macrophage-activating factor.小鼠干扰素-γ与巨噬细胞激活因子的生物学及抗原相似性
J Exp Med. 1984 Mar 1;159(3):812-27. doi: 10.1084/jem.159.3.812.
5
Antiviral effects of bacteria-derived human leukocyte interferons against encephalomyocarditis virus infection of squirrel monkeys. Brief report.细菌衍生的人白细胞干扰素对松鼠猴脑心肌炎病毒感染的抗病毒作用。简要报告。
Arch Virol. 1983;76(4):365-72. doi: 10.1007/BF01311204.
6
Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.人α-2干扰素基因的化学合成及其在大肠杆菌中的表达。
Nucleic Acids Res. 1983 Sep 24;11(18):6419-35. doi: 10.1093/nar/11.18.6419.
7
Cloning and expression of murine immune interferon cDNA.小鼠免疫干扰素cDNA的克隆与表达
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5842-6. doi: 10.1073/pnas.80.19.5842.
8
Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli.通过大肠杆菌中相关克隆基因之间的重组形成编码杂合蛋白的基因。
Nucleic Acids Res. 1983 Aug 25;11(16):5661-9. doi: 10.1093/nar/11.16.5661.
9
Orientation of a human leukocyte interferon molecule on its cell surface receptor: carboxyl terminus remains accessible to a monoclonal antibody made against a synthetic interferon fragment.人白细胞干扰素分子在其细胞表面受体上的取向：羧基末端对于针对合成干扰素片段制备的单克隆抗体仍可及。
Proc Natl Acad Sci U S A. 1983 May;80(9):2539-43. doi: 10.1073/pnas.80.9.2539.
10
Antiviral and protein-inducing activities of recombinant human leukocyte interferons and their hybrids.重组人白细胞干扰素及其杂种的抗病毒和诱导蛋白活性
J Virol. 1984 May;50(2):445-50. doi: 10.1128/JVI.50.2.445-450.1984.

本文引用的文献

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Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
2
Characterization of a subspecies of mouse interferon cross-reactive on human cells and antigenically related to human leukocyte interferon.一种对人类细胞具有交叉反应且与人类白细胞干扰素抗原相关的小鼠干扰素亚种的特性分析。
Virology. 1980 Feb;101(1):315-8. doi: 10.1016/0042-6822(80)90512-7.
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Properties of a human alpha-interferon purified from E. coli extracts.从大肠杆菌提取物中纯化得到的人α干扰素的特性。
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4
Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them.在大肠杆菌中产生的两种人α干扰素及由它们衍生的杂交分子的靶细胞特异性。
Proc Natl Acad Sci U S A. 1981 May;78(5):2848-52. doi: 10.1073/pnas.78.5.2848.
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DNA sequence of two closely linked human leukocyte interferon genes.两个紧密连锁的人白细胞干扰素基因的DNA序列
Science. 1981 Jun 5;212(4499):1159-62. doi: 10.1126/science.6165082.
6
Bacterial synthesis of a novel human leukocyte interferon.一种新型人白细胞干扰素的细菌合成。
Nucleic Acids Res. 1981 Feb 11;9(3):731-41. doi: 10.1093/nar/9.3.731.
7
Dissociation of interferon effects on murine leukemia virus and encephalomyocarditis virus replication in mouse cells.干扰素对小鼠细胞中鼠白血病病毒和脑心肌炎病毒复制影响的解离
J Virol. 1981 Feb;37(2):827-31. doi: 10.1128/JVI.37.2.827-831.1981.
8
The structure of eight distinct cloned human leukocyte interferon cDNAs.八个不同克隆的人白细胞干扰素cDNA的结构。
Nature. 1981 Mar 5;290(5801):20-6. doi: 10.1038/290020a0.
9
Assignment of the disulphide bonds of leukocyte interferon.白细胞干扰素二硫键的确定
Nature. 1981 Feb 12;289(5798):606-7. doi: 10.1038/289606a0.
10
Synthesis of human fibroblast interferon by E. coli.大肠杆菌合成人成纤维细胞干扰素。
Nucleic Acids Res. 1980 Sep 25;8(18):4057-74. doi: 10.1093/nar/8.18.4057.

两种主要人类白细胞干扰素杂交体的抗病毒活性

Antiviral activities of hybrids of two major human leukocyte interferons.

作者信息

Weck P K, Apperson S, Stebbing N, Gray P W, Leung D, Shepard H M, Goeddel D V

出版信息

Nucleic Acids Res. 1981 Nov 25;9(22):6153-66. doi: 10.1093/nar/9.22.6153.

DOI:10.1093/nar/9.22.6153
PMID:6171779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327591/
Abstract

Four hybrid human leukocyte interferon (LeIF or IFN-alpha) genes have been constructed by in vitro recombination of LeIF-A (IFN-alpha 2) and LeIF-D (IFN-alpha 1) genes at common restriction endonuclease sites located within their coding regions. These hybrid genes have been expressed in E. coli under trp promoter control. The interferons produced [LeIF-AD (BglII), -AD (PvuII), -DA (BglII), -DA (PvuII)] have antiviral properties distinct from the parental molecules LeIF-A and -D, varying considerably in their abilities to inhibit plaque formation by different viruses in a range of mammalian cells. All six of the cloned LeIFs exhibit the heat stability, pH 2 stability and antigenic specificity of natural leukocyte interferons.

摘要

通过在位于其编码区域内的常见限制性内切酶位点对LeIF-A（IFN-α2）和LeIF-D（IFN-α1）基因进行体外重组，构建了四个杂交人白细胞干扰素（LeIF或IFN-α）基因。这些杂交基因已在色氨酸启动子控制下在大肠杆菌中表达。所产生的干扰素[LeIF-AD（BglII）、-AD（PvuII）、-DA（BglII）、-DA（PvuII）]具有与亲本分子LeIF-A和-D不同的抗病毒特性，在一系列哺乳动物细胞中抑制不同病毒形成蚀斑的能力差异很大。所有六个克隆的LeIF都表现出天然白细胞干扰素的热稳定性、pH 2稳定性和抗原特异性。