Bartsch W, Krieg M, Becker H, Mohrmann J, Voigt K D
Acta Endocrinol (Copenh). 1982 Aug;100(4):634-40. doi: 10.1530/acta.0.1000634.
5 alpha-Dihydrotestosterone (DHT) and 5 alpha-androstane-3 alpha, 18 beta-diol (3 alpha-diol) were extracted from epithelium and stroma of human benign prostatic hyperplasia (BPH) and of normal prostate and quantified by RIA. The main results were: (1) concerning the BPH, DHT is mainly located in the nuclear fraction of epithelium and stroma, whereas 3 alpha-diol was completely of extranuclear origin, (2) in the nuclei derived from BPH stroma the DHT content (7.1 +/- 0.88 pmol/mg DNA, mean +/- SEM [n = 14]) was significantly higher (P less than 0.01) than in the nuclei derived from BPH epithelium (3.8 +/- 0.38 pmol/mg DNA [n = 14]). The DHT content in the nuclear fraction derived from unseparated, i.e. whole tissue, was 5.6 +/- 0.60 pmol/mg DNA [n =14], (3) the biological significance of the overwhelming DHT accumulation in the stromal nuclei for the BPH tissue is reflected by a significant correlation between the DHT values in the nuclei from stroma and whole tissue (rs = 0.710, P less than 0.01) and (4) in four normal prostates the DHT content in the nuclear fraction of epithelium (1.3 +/- 0.37 pmol/mg DNA) and stroma (2.2 +/- 0.93 pmol/mg DNA) was significantly lower (P less than 0.01, each) compared with the respective BPH fraction. These data support earlier findings which indicate that the stroma of BPH is a preferential tissue for androgen metabolism.
从人良性前列腺增生（BPH）及正常前列腺的上皮和基质中提取5α-双氢睾酮（DHT）和5α-雄甾烷-3α，18β-二醇（3α-二醇），并用放射免疫分析法进行定量。主要结果如下：（1）关于BPH，DHT主要位于上皮和基质的核部分，而3α-二醇完全来自核外；（2）在BPH基质来源的细胞核中，DHT含量（7.1±0.88 pmol/mg DNA，均值±标准误[n = 14]）显著高于（P<0.01）BPH上皮来源的细胞核（3.8±0.38 pmol/mg DNA [n = 14]）。未分离的即整个组织来源的核部分中DHT含量为5.6±0.60 pmol/mg DNA [n =14]；（3）基质细胞核中大量DHT积累对BPH组织的生物学意义体现在基质细胞核与整个组织中DHT值之间存在显著相关性（rs = 0.710，P<0.01）；（4）在4个正常前列腺中，上皮（1.3±0.37 pmol/mg DNA）和基质（2.2±0.93 pmol/mg DNA）核部分中的DHT含量与相应的BPH部分相比均显著降低（均为P<0.01）。这些数据支持了早期的研究结果，即BPH的基质是雄激素代谢的优先组织。
