Bice D E, Muggenburg B A
Inhalation Toxicology Research Institute, Albuquerque, NM 87185, USA.
Immunology. 1996 Jun;88(2):191-7. doi: 10.1111/j.1365-2567.1996.tb00004.x.
In comparison to primary immune responses after lung immunization, the level of antigen-specific antibody and the number of cells producing specific antibody are significantly increased after challenging the lungs with antigen. The response of immune memory cells in the lung to an antigen challenge could be responsible for this elevated immune response. However, increased numbers of antibody-producing cells, possibly produced in the lung-associated lymph nodes, are also found in the blood after an antigen challenge. Therefore, it is possible that both the response of immune memory cells in the lung, and the recruitment of antibody-producing cells from the blood, contribute to the elevated levels of antibody in the lung after an antigen challenge. This study compared the level of antibody produced in the lung by the response of pulmonary immune memory cells with the level of antibody produced by antibody-forming cells that enter the lung from blood after an antigen challenge. This comparison was made possible by immunizing and challenging two lung lobes of dogs with two antigens. The immune responses to both antigens were then evaluated in both lung lobes after primary immunization and challenge. Data from these evaluations showed that most antibody in the lung after an antigen challenge is produced by a localized anamnestic response of pulmonary immune memory cells. A significantly lower level of antibody entered the lung from the vasculature and/or was produced by antibody-forming cells that entered the lung from blood after an antigen challenge.
与肺部免疫后的初次免疫反应相比，在用抗原攻击肺部后，抗原特异性抗体水平和产生特异性抗体的细胞数量显著增加。肺部免疫记忆细胞对抗原攻击的反应可能是这种增强的免疫反应的原因。然而，在用抗原攻击后，血液中也发现了数量增加的、可能在肺相关淋巴结中产生的抗体产生细胞。因此，肺部免疫记忆细胞的反应以及从血液中募集抗体产生细胞，都有可能导致抗原攻击后肺部抗体水平的升高。本研究比较了肺部免疫记忆细胞反应在肺部产生的抗体水平与抗原攻击后从血液进入肺部的抗体形成细胞产生的抗体水平。通过用两种抗原免疫和攻击犬的两个肺叶，使得这种比较成为可能。然后在初次免疫和攻击后，对两个肺叶中针对两种抗原的免疫反应进行评估。这些评估数据表明，抗原攻击后肺部的大多数抗体是由肺部免疫记忆细胞的局部回忆反应产生的。从血管系统进入肺部和/或由抗原攻击后从血液进入肺部的抗体形成细胞产生的抗体水平显著较低。
