Esquivel C O, Bergqvist D, Björck C G, Nilsson B, Bergentz S E
Acta Chir Scand. 1982;148(4):359-62.
The lysability of ex vivo thrombi before and after the infusion of dextran 70 (0.5 g/kg, 1.5 g/kg and 2 g/kg), dextran 40 (1.5 g/kg), albumin 5% (1.5 g/kg) and normal saline (0.3 g/kg) was studied in rabbits. All rabbits received 125I fibrinogen before the experiment. The thrombi were treated with plasmin or sodium chloride and the radioactivity released in the supernatant by the thrombus was determined. The thrombus weight was also recorded. A gradual increase in the lysability of thrombi after dextran 70 administration was observed and the maximum effect occurred 24 hours later. Increasing dose of dextran 70 did not potentiate the release of 125I fibrin degradation products in the supernatant, but did significantly decrease the thrombus weight during the first hours postinfusion, Dextran 40 was found to have a similar action but the effect was shorter and the lysability at 24 hours was similar to the preinfusion values. Albumin 5% and normal saline increase the radioactivity released from the thrombi to the supernatant but no change in the thrombus weight was observed. A similar result was obtained in the control group. The present investigation demonstrated that increasing doses of dextran 70 potentiate the effect on thrombolysis demonstrated by statistically significant decrease in the thrombus weight. Furthermore, albumin 5% and normal saline increase the release of 125I fibrin split products in the supernatant but did not have any effect on the thrombus weight and finally, the rabbit showed a marked spontaneous fibrinolysis probably related to the stress, anesthetic agent and surgical manipulation.
在兔体内研究了输注右旋糖酐70（0.5g/kg、1.5g/kg和2g/kg）、右旋糖酐40（1.5g/kg）、5%白蛋白（1.5g/kg）和生理盐水（0.3g/kg）前后离体血栓的溶解能力。所有兔子在实验前均接受125I纤维蛋白原。血栓用纤溶酶或氯化钠处理，并测定血栓在上清液中释放的放射性。同时记录血栓重量。观察到给予右旋糖酐70后血栓溶解能力逐渐增强，最大效应在24小时后出现。增加右旋糖酐70的剂量并未增强上清液中125I纤维蛋白降解产物的释放，但在输注后的最初几小时内确实显著降低了血栓重量。发现右旋糖酐40有类似作用，但效果持续时间较短，24小时时的溶解能力与输注前值相似。5%白蛋白和生理盐水增加了血栓向上清液中释放的放射性，但未观察到血栓重量的变化。对照组也得到了类似结果。本研究表明，增加右旋糖酐70的剂量可增强溶栓作用，表现为血栓重量有统计学意义的下降。此外，5%白蛋白和生理盐水增加了上清液中125I纤维蛋白裂解产物的释放，但对血栓重量没有任何影响，最后，兔子表现出明显的自发纤溶，这可能与应激、麻醉剂和手术操作有关。
