Bettelheim P, Paietta E, Majdic O, Gadner H, Schwarzmeier J, Knapp W
Blood. 1982 Dec;60(6):1392-6.
The expression of a myeloid-specific antigen was detected on TdT-positive blast cell populations in two cases of childhood acute lymphocytic leukemia. Double-fluorescence staining by using the monoclonal antibody, VIM-D5, which is specific for cells of myeloid origin, in combination with TdT antiserum revealed that a distinct portion of the blast cells carried both markers. The finding represents the first direct demonstration of this specific biphenotype in leukemic cells and was interpreted as the abnormal expression of a myeloid antigen on lymphoid blast cells.
在两例儿童急性淋巴细胞白血病中，在末端脱氧核苷酸转移酶（TdT）阳性的原始细胞群体中检测到一种髓系特异性抗原的表达。使用对髓系来源细胞具有特异性的单克隆抗体VIM-D5与TdT抗血清进行双重荧光染色，结果显示有一部分原始细胞同时携带这两种标志物。这一发现首次直接证明了白血病细胞中这种特定的双表型，并被解释为淋巴样原始细胞上髓系抗原的异常表达。
