Wakizaka A, Okuhara E
J Biochem. 1982 Sep;92(3):757-63. doi: 10.1093/oxfordjournals.jbchem.a133987.
Pyrimidine, purine, and mixed sequence oligonucleotides from ultraviolet-irradiated DNA were tested for their inhibitory activities on the interaction of [3H]ultraviolet-irradiated DNA with its antibody raised in rabbit. Thymine dimer containing pyrimidine oligonucleotides from irradiated DNA failed to inhibit the interaction, while mixed sequence oligonucleotides, especially those with 8 or more nucleotides, exhibited potent inhibition. Purine clusters from irradiated DNA and mixed sequence oligomers from unirradiated DNA showed no inhibition. Dimerized thymine, which appears to be a critical part of the antigenic determinant, did not inhibit the interaction by itself. The same observations were made for ultraviolet-irradiated thymidine and thymidylic acid. The results suggest that a structure composed of a mixed pyrimidine and purine sequence with a certain chain length seems to be essential for the antigenicity induced in the irradiated DNA. On this nucleotide chain backbone, photochemically modified bases (mostly thymine dimer) can form an immune-active structure.
对来自紫外线照射DNA的嘧啶、嘌呤和混合序列寡核苷酸，测试了它们对[³H]紫外线照射DNA与其在兔体内产生的抗体之间相互作用的抑制活性。来自照射DNA的含胸腺嘧啶二聚体的嘧啶寡核苷酸未能抑制这种相互作用，而混合序列寡核苷酸，尤其是那些含有8个或更多核苷酸的寡核苷酸，表现出强效抑制作用。来自照射DNA的嘌呤簇和来自未照射DNA的混合序列寡聚物没有显示出抑制作用。二聚化的胸腺嘧啶似乎是抗原决定簇的关键部分，但它本身并不抑制这种相互作用。对紫外线照射的胸苷和胸苷酸也有相同的观察结果。结果表明，由具有一定链长的嘧啶和嘌呤混合序列组成的结构似乎是照射DNA诱导抗原性所必需的。在这种核苷酸链主链上，光化学修饰的碱基（主要是胸腺嘧啶二聚体）可以形成免疫活性结构。
