Wakizaka A, Okuhara E
J Biochem. 1979 Nov;86(5):1469-78. doi: 10.1093/oxfordjournals.jbchem.a132665.
The conformational changes of double-stranded DNA induced during irradiation with ultraviolet, were immunologically investigated. These studies revealed that at least two distinct antigenic sites were induced in the irradiated DNA molecule, giving rise to two different antibodies specific for ultraviolet-irradiated (uv) DNA and thermally denatured DNA-like structure, and these were demonstrable using radioimmunoassay and double diffusion tests. A series of experiments, including melting profile, fluorescence intensity of the ethidium bromide complex and chromatographic behavior on hydroxyapatite, performed on the antigenically active uvDNA indicated that the duplex structure of DNA separated irregularly during irradiation. Furthermore, the data showed that the conformational determinants of uvDNA are located on the exposed single-stranded regions.
对紫外线照射过程中双链DNA诱导产生的构象变化进行了免疫学研究。这些研究表明，在受照射的DNA分子中至少诱导出两个不同的抗原位点，产生了两种针对紫外线照射（uv）DNA和热变性DNA样结构的不同抗体，并且可以使用放射免疫测定和双向扩散试验进行检测。对具有抗原活性的uvDNA进行的一系列实验，包括熔解曲线、溴化乙锭复合物的荧光强度和在羟基磷灰石上的色谱行为，表明DNA的双链结构在照射过程中不规则地分离。此外，数据显示uvDNA的构象决定簇位于暴露的单链区域。
