Morito T, Kano K, Milgrom F
J Immunol. 1982 Dec;129(6):2524-8.
An enzyme immunoassay (EIA) was developed for the detection of heterophile, Hanganutziu-Deicher (H-D) antibodies in sera of patients with infectious mononucleosis (IM) and various other diseases. The EIA with a high m.w. glycoprotein (HMWGP) isolated from bovine erythrocyte stromata was shown to detect H-D antibodies directly, in spite of higher titers of Paul-Bunnell (P-B) antibodies in the IM sera. Absorption and inhibition studies of IM sera demonstrated H-D specificity of the antibodies combining with HMWGP in the EIA. The H-D antibodies were found in sera of 56% Caucasians and 27% of Japanese suffering from IM. The vast majority of the H-D antibodies in IM sera was of IgM class. Sera of patients with various other diseases also gave positive results: rheumatoid arthritis, 22%; syphilis, 19%; cancer of the gastrointestinal tract, 13%; and lepromatous leprosy 9.7%. The incidence of positive results in control sera from apparently healthy subjects was less than 4%. Results of this study confirmed our previous observation that whereas P-B antigens appear in immunogenic form in only IM, the H-D antigen is expressed as an immunogen in various diseases including IM.
已开发出一种酶免疫测定法（EIA），用于检测传染性单核细胞增多症（IM）患者及其他多种疾病患者血清中的嗜异性杭-德（H-D）抗体。尽管IM血清中保罗-邦内尔（P-B）抗体效价较高，但从牛红细胞基质中分离出的高分子量糖蛋白（HMWGP）的EIA仍能直接检测H-D抗体。对IM血清的吸收和抑制研究表明，在EIA中与HMWGP结合的抗体具有H-D特异性。在患IM的56%的白种人和27%的日本人的血清中发现了H-D抗体。IM血清中绝大多数H-D抗体为IgM类。患有其他各种疾病的患者血清也呈阳性结果：类风湿性关节炎，22%；梅毒，19%；胃肠道癌，13%；瘤型麻风，9.7%。明显健康受试者的对照血清中阳性结果的发生率低于4%。本研究结果证实了我们之前的观察，即虽然P-B抗原仅在IM中以免疫原形式出现，但H-D抗原在包括IM在内的多种疾病中均作为免疫原表达出来。
