Sommé G, Serra J R, Leclercq L, Moreau J L, Mazie J C, Moinier D, Fougerèau M, Thèze J
Mol Immunol. 1982 Aug;19(8):1011-9. doi: 10.1016/0161-5890(82)90309-1.
The contribution of the H- and L-chains to the structure of the main idiotype of anti-poly (Glu60-Ala30-Tyr10) (GAT) antibodies has been studied. This idiotype has been previously divided into four types of specificity: (1) the highly conserved idiotypic specificity (h.c. GAT) is expressed by anti-GAT antibodies from the guinea-pig, rat and mice; (2) the public specificity (p. GAT) is expressed in an identical form by all anti-GAT antibodies from all strains of mice tested and by all hybridoma products (HP) with anti-GAT activity; (3) the strain-restricted specificity (s.r. GAT-1) is only expressed by anti-GAT antibodies from strains with Ig-1a, Ig-1c and Ig-1c allotypic markers; and finally (4) the individual specificity i1-GAT defined on HP G5 is also expressed by most of the hybridoma protein with anti-poly (Glu50-Tyr50) (GT) activity. In this paper we show that h.c.GAT, p.GAT and i1-GAT require the interaction of H- and L-chains to be expressed: (1) isolated H- and L-chains from HP G5 did not express these specificities; and (2) recombinant molecules composed of H- and L-chains from HP with anti-GAT activity and an irrelevant myeloma protein (MOPC21) never expressed h.c.GAT, p.GAT and i1-GAT. We next investigated the relationship between the GAT binding site and the p.GAT, h.c.GAT and s.r.GAT-1 idiotypic specificities. GAT and GT were not able to inhibit the binding to s.r.GAT-1 while they inhibit the idiotypic binding to p.GAT and h.c.GAT. A GAT fragment of mol. wt 3000 was also shown to inhibit the binding of p.GAT and h.c.GAT to the appropriate sera. Thus p.GAT and h.c.GAT are very close to the GAT combining site while s.r.GAT-1 represents an idiotypic specificity located outside the GAT binding site.
已对重链和轻链在抗聚（Glu60 - Ala30 - Tyr10）（GAT）抗体主要独特型结构中的作用进行了研究。此前已将这种独特型分为四种特异性类型：（1）高度保守的独特型特异性（h.c. GAT）由豚鼠、大鼠和小鼠的抗GAT抗体表达；（2）共有特异性（p. GAT）由所有测试小鼠品系的所有抗GAT抗体以及所有具有抗GAT活性的杂交瘤产物（HP）以相同形式表达；（3）品系限制特异性（s.r. GAT - 1）仅由具有Ig - 1a、Ig - 1c和Ig - 1c同种异型标记的品系的抗GAT抗体表达；最后（4）在HP G5上定义的个体特异性i1 - GAT也由大多数具有抗聚（Glu50 - Tyr50）（GT）活性的杂交瘤蛋白表达。在本文中，我们表明h.c.GAT、p.GAT和i1 - GAT需要重链和轻链相互作用才能表达：（1）从HP G5分离的重链和轻链不表达这些特异性；（2）由具有抗GAT活性的HP的重链和轻链与无关骨髓瘤蛋白（MOPC21）组成的重组分子从未表达h.c.GAT、p.GAT和i1 - GAT。接下来，我们研究了GAT结合位点与p.GAT、h.c.GAT和s.r.GAT - 1独特型特异性之间的关系。GAT和GT不能抑制与s.r.GAT - 1的结合，而它们能抑制与p.GAT和h.c.GAT的独特型结合。一个分子量为3000的GAT片段也被证明能抑制p.GAT和h.c.GAT与相应血清的结合。因此，p.GAT和h.c.GAT非常接近GAT结合位点，而s.r.GAT - 1代表位于GAT结合位点之外的独特型特异性。
