Carter M J, Willcocks M M, Löffler S, ter Meulen V
J Gen Virol. 1982 Nov;63 (Pt 1):113-20. doi: 10.1099/0022-1317-63-1-113.
Twenty-one monoclonal antibodies directed against the measles virus haemagglutinin have recently been obtained. These were known to fall into five groups, each defined by its effects on the biological functions of the H protein. A representative of each group was selected and examined by competitive radioimmunoassay in an attempt to deduce the relationships between antibody-binding sites on the antigen. It was found that these five antibodies formed three binding groups which recognized different but overlapping areas of the molecule. These three areas formed a series of sites which traversed the active region of the H polypeptide. A haemagglutinin-directed monoclonal antibody which displayed a haemolysin-inhibiting activity was also examined. This antibody fitted into the binding group scheme determined here and there was no additional binding site for this molecule on the F protein itself.
最近获得了21种针对麻疹病毒血凝素的单克隆抗体。已知这些抗体可分为五组，每组由其对H蛋白生物学功能的影响来定义。从每组中挑选出一个代表，并通过竞争性放射免疫测定法进行检测，以推断抗原上抗体结合位点之间的关系。结果发现，这五种抗体形成了三个结合组，它们识别分子上不同但重叠的区域。这三个区域形成了一系列贯穿H多肽活性区域的位点。还检测了一种具有溶血抑制活性的血凝素导向单克隆抗体。该抗体符合此处确定的结合组模式，并且在F蛋白本身没有该分子的额外结合位点。
