ATP酶相关抗原（M2）：原发性胆汁性肝硬化血清学诊断的标志物抗原。

ATPase-associated antigen (M2): marker antigen for serological diagnosis of primary biliary cirrhosis.

作者信息

Berg P A, Klein R, Lindenborn-Fotinos J, Klöppel W

出版信息

Lancet. 1982 Dec 25;2(8313):1423-6. doi: 10.1016/s0140-6736(82)91327-7.

Abstract

Serum samples from 94 patients with primary biliary cirrhosis (PBC) and 17 patients with chronic cholestatic hepatitis (CCH) were tested in the fluorometric immunoassay (FIAX) against the nonorgan-specific ATPase-associated antigen (M2) and against submitochondrial from beef heart and rat liver, to evaluate the specificity and sensitivity of the M2 antigen for the diagnosis of PBC. As controls serum samples from 42 patients with other antimitochondrial antibody (AMA) specificity (against M1, M3, M5, and M6) as well as samples from 417 patients with various other hepatic and non-hepatic disorders were used. Serum samples from 91 of the 94 PBC patients (97%) and all 17 with CCH reacted with the M2 antigen. However, when SMP from rat liver and beef heart were tested in parallel in the FIAX, AMA could be detected in all PBC serum samples. None of the 42 patients with different types of AMA had reactions with the M2 antigen but all had reactions with SMP from rat-liver or beef-heart mitochondria or both. Among the other 417 patients with hepatic and non-hepatic disorders only 4(1%), all with collagen diseases, had anti-M2 antibodies.

摘要

采用荧光免疫分析法（FIAX），对94例原发性胆汁性肝硬化（PBC）患者和17例慢性胆汁淤积性肝炎（CCH）患者的血清样本进行检测，以检测非器官特异性ATP酶相关抗原（M2）以及牛心和大鼠肝脏的亚线粒体，从而评估M2抗原对PBC诊断的特异性和敏感性。作为对照，使用了42例具有其他抗线粒体抗体（AMA）特异性（针对M1、M3、M5和M6）患者的血清样本以及417例患有各种其他肝脏和非肝脏疾病患者的样本。94例PBC患者中的91例（97%）以及所有17例CCH患者的血清样本均与M2抗原发生反应。然而，当在FIAX中同时检测大鼠肝脏和牛心的亚线粒体时，在所有PBC血清样本中均可检测到AMA。42例具有不同类型AMA的患者中，无一例与M2抗原发生反应，但所有患者均与大鼠肝脏或牛心线粒体的亚线粒体或两者均发生反应。在其他417例患有肝脏和非肝脏疾病的患者中，只有4例（1%）患有抗M2抗体，且均患有胶原病。