Desnoyers M M, Haines D M, Searcy G P
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon.
Can J Vet Res. 1990 Jun;54(3):360-5.
Normal and well differentiated neoplastic canine tissues were immunohistochemically stained for keratin, vimentin and desmin intermediate filament proteins using commercially available monoclonal antibodies. Keratin was detected in 56 of 57 carcinomas, vimentin in 59 of 62 sarcomas and desmin in three of four muscle cell tumors. Most normal and neoplastic tissues expressed only one type of intermediate filament; exceptions were one hemangiosarcoma and one pulmonary carcinoma in which there was coexpression of vimentin and keratin proteins. Since immunohistochemical detection of intermediate filaments has tissue-specific distribution in the majority of well differentiated canine neoplasms, these stains may be useful in the differential diagnosis of anaplastic canine tumors. However, the monoclonal antibodies to cytokeratin which were tested in this study failed to detect intermediate filaments in liver, pancreas and salivary glands which suggests that these antibodies may also be unable to detect epithelial tumors derived from these tissues. In addition, in nine neoplasms, the normal tissues adjacent to neoplastic cells failed to stain for the intermediate filament normally expressed. When this occurs, evaluation of intermediate filament expression is invalid for the determination of tissue of origin of the neoplastic cells.
使用市售单克隆抗体，对正常和高分化的犬肿瘤组织进行角蛋白、波形蛋白和结蛋白中间丝蛋白的免疫组织化学染色。在57例癌中有56例检测到角蛋白，62例肉瘤中有59例检测到波形蛋白，4例肌肉细胞瘤中有3例检测到结蛋白。大多数正常和肿瘤组织仅表达一种类型的中间丝；例外情况是1例血管肉瘤和1例肺癌，其中波形蛋白和角蛋白蛋白共表达。由于中间丝的免疫组织化学检测在大多数高分化犬肿瘤中具有组织特异性分布，这些染色可能有助于间变性犬肿瘤的鉴别诊断。然而，本研究中测试的细胞角蛋白单克隆抗体未能在肝脏、胰腺和唾液腺中检测到中间丝，这表明这些抗体可能也无法检测源自这些组织的上皮肿瘤。此外，在9个肿瘤中，肿瘤细胞相邻的正常组织未能对正常表达的中间丝染色。当出现这种情况时，中间丝表达的评估对于确定肿瘤细胞的组织来源无效。
