Sorrentino V, Di Francesco P, Soria M, Rossi G B
J Gen Virol. 1982 Dec;63(2):509-11. doi: 10.1099/0022-1317-63-2-509.
A line of human amniotic cells (UAC) was found to yield large amounts of fibroblast interferon (IFN-beta; 100 to 150 IU/10(3) cells) upon induction with Newcastle disease virus (NDV). UAC cells have a doubling time of about 24 h, and do not require foetal calf serum for growth or optimal IFN yield. The IFN produced was shown to be HuIFN-beta by assaying it on homologous and heterologous cells, and by neutralization tests with specific antisera. It could be purified to a specific activity of 0 . 6 X 10(7) IU/mg protein by chromatography on Blue Sepharose. Addition of 8 micrograms poly(A+) RNA from NDV-induced UAC in 100 microliter of reticulocyte lysate resulted in the production of 510 +/- 340 IU/ml of an IFN that was neutralized only by anti-HuIFN-beta serum.
发现一株人羊膜细胞（UAC）在用新城疫病毒（NDV）诱导后能产生大量成纤维细胞干扰素（IFN-β；100至150国际单位/10³个细胞）。UAC细胞的倍增时间约为24小时，生长或获得最佳干扰素产量不需要胎牛血清。通过在同源和异源细胞上进行测定以及用特异性抗血清进行中和试验，证明所产生的干扰素为HuIFN-β。通过在蓝色琼脂糖凝胶上进行层析，可将其纯化至比活性为0.6×10⁷国际单位/毫克蛋白质。向100微升网织红细胞裂解物中添加来自NDV诱导的UAC的8微克聚腺苷酸（poly(A+)）RNA，导致产生510±340国际单位/毫升的干扰素，该干扰素仅被抗HuIFN-β血清中和。
