Yanaura S, Takeda H, Misawa M
J Pharmacobiodyn. 1982 Aug;5(8):603-10. doi: 10.1248/bpb1978.5.603.
The effects of L-cysteine methyl ester on behavior of mucus glycoproteins in canine tracheal secretory cells were investigated histologically and histochemically. Low concentrations (10(-7) and 10(-6)M) of L-cysteine methyl ester produced, as histological changes, decreases in the total goblet cell number and the thickness of the acini of submucosal glands, and an increase in the acinar inner diameter. On the other hand, L-cysteine methyl ester at 10(-5) and 10(-4)M produced an increase in the thickness of the acini and a slight decrease in the acinar inner diameter. Both the total acid glycoprotein and sulphated glycoprotein contents in glandular cells markedly decreased, whereas the neutral glycoprotein content in the cells increased following L-cysteine methyl ester treatment in a concentration-dependent way. Total saccharide and protein concentrations in the incubation fluid increased with 10(-7) and 10(-6)M, but decreased with 10(-5) and 10(-4)M L-cysteine methyl ester. N-Acetylhexosamine concentration in the bathing fluid increased at 10(-7) and 10(-6)M of the agent. These findings suggest that low concentrations of L-cysteine methyl ester stimulate secretory activities of tracheal secretory cells, while at high concentrations the agent stimulates synthesis of mucus glycoproteins in the submucosal glands. Additionally, it is suggested that L-cysteine methyl ester has a marked viscosity lowering effect on the mucus in the secretory cells.
采用组织学和组织化学方法研究了L-半胱氨酸甲酯对犬气管分泌细胞中黏液糖蛋白行为的影响。低浓度（10⁻⁷和10⁻⁶M）的L-半胱氨酸甲酯产生的组织学变化包括杯状细胞总数减少、黏膜下腺腺泡厚度减小以及腺泡内径增加。另一方面，10⁻⁵和10⁻⁴M的L-半胱氨酸甲酯导致腺泡厚度增加和腺泡内径略有减小。经L-半胱氨酸甲酯处理后，腺细胞中的总酸性糖蛋白和硫酸化糖蛋白含量显著降低，而细胞中的中性糖蛋白含量则呈浓度依赖性增加。培养液中的总糖和蛋白质浓度在10⁻⁷和10⁻⁶M时增加，但在10⁻⁵和10⁻⁴M的L-半胱氨酸甲酯时降低。在10⁻⁷和10⁻⁶M的该试剂作用下，浴液中的N-乙酰己糖胺浓度增加。这些发现表明，低浓度的L-半胱氨酸甲酯刺激气管分泌细胞的分泌活动，而高浓度时该试剂刺激黏膜下腺中黏液糖蛋白的合成。此外，提示L-半胱氨酸甲酯对分泌细胞中的黏液具有显著的降低黏度作用。
