Yanaura S, Takeda H, Misawa M
Nihon Yakurigaku Zasshi. 1982 Jan;79(1):57-64.
The effects of glyceryl guaiacolate on secretory activities of tracheal secretory cells and on behavior of mucus glycoprotein in these cells were investigated histologically and histochemically using isolated canine trachea. Following glyceryl guaiacolate treatment, the number of total glycoprotein-containing goblet cells (GC) did not change. The numbers of acid glycoprotein (AGP)-, neutral glycoprotein (NGP)-, and sulphated glycoprotein (SGP)-containing GC were also unaltered in a concentration range of 10(-7) to 10(-4)M. On the other hand the acinar inner diameter of the submucosal gland (SG) and the acinar inner diameter to wall ratio significantly increased, while thickness of acinus significantly decreased with 10(-6), 10(-5) and 10(-4)M glyceryl guaiacolate. AGP and SGP contents in glandular cells markedly decreased, while the ratio of the numbers of AGP- to NGP-containing glandular cell were the same, suggesting that glyceryl guaiacolate does not change the quality of mucus glycoprotein in GC and SG. Glyceryl guaiacolate produced a marked increase in total saccharide, protein, and N-acetylhexosamine concentrations in the incubation fluid. These findings suggest that glyceryl guaiacolate has no influence on the secretory activity of GC, but markedly stimulates the activity of SG. The secretagogue effect of the agent would be ascribable to stimulation of mucus discharge, but not stimulation of mucus synthesis.
使用离体犬气管，通过组织学和组织化学方法研究了愈创木酚甘油醚对气管分泌细胞分泌活动以及这些细胞中黏液糖蛋白行为的影响。用愈创木酚甘油醚处理后，含总糖蛋白的杯状细胞（GC）数量未发生变化。在10⁻⁷至10⁻⁴M的浓度范围内，含酸性糖蛋白（AGP）、中性糖蛋白（NGP）和硫酸化糖蛋白（SGP）的GC数量也未改变。另一方面，10⁻⁶、10⁻⁵和10⁻⁴M愈创木酚甘油醚使黏膜下腺（SG）的腺泡内径以及腺泡内径与壁的比值显著增加，而腺泡厚度显著减小。腺细胞中的AGP和SGP含量明显降低，而含AGP与含NGP腺细胞数量的比值相同，这表明愈创木酚甘油醚不会改变GC和SG中黏液糖蛋白的质量。愈创木酚甘油醚使孵育液中的总糖、蛋白质和N - 乙酰己糖胺浓度显著增加。这些发现表明，愈创木酚甘油醚对GC的分泌活动没有影响，但能显著刺激SG的活动。该药物的促分泌作用可能归因于对黏液排出的刺激，而非对黏液合成的刺激。
