Knapp L W, O'Guin W M, Sawyer R H
Science. 1983 Feb 4;219(4584):501-3. doi: 10.1126/science.6186022.
The distribution of keratin intermediate filaments, previously considered static in organization and imperturbable by conventional drugs used to alter the structure and organization of the cytoskeleton, can be altered significantly by treatment with colchicine and cytochalasin D. The loss of microfilaments and microtubules converts the keratin cytoskeleton from a branching, even distribution to a series of starlike structures whose filaments are maintained by multiple membrane attachment sites. These findings provide a means for manipulating cytokeratin organization to investigate the role of keratins in cytoskeletal structure and function.
角蛋白中间丝的分布，以前被认为在组织结构上是静态的，并且不会被用于改变细胞骨架结构和组织的传统药物所扰动，但用秋水仙碱和细胞松弛素D处理后，其分布会发生显著改变。微丝和微管的缺失将角蛋白细胞骨架从分支状的均匀分布转变为一系列星状结构，其细丝由多个膜附着位点维持。这些发现为操纵细胞角蛋白组织以研究角蛋白在细胞骨架结构和功能中的作用提供了一种方法。
