McDonald I, Rose N R, Pontes E J, Choe B K
Arch Androl. 1978 May;1(3):235-9. doi: 10.3109/01485017808988342.
When serial dilution of standard prostatic acid phosphatases (PAP) was reacted with constant amounts of anti-PAP serum by counterimmunoelectrophoresis (CIEP), the detection end point of enzyme concentration was 0.25 ng in a 10 microliter sample volume. The PAP concentrations in unknowns can be quantitated by comparing the dilution end points of reference PAP with the testing samples. Serum PAP levels were determined by a radioimmunoassay (RIA) and CIEP using normal male and female sera and serum samples from patients with prostatic cancer and nonprostatic tumors. An excellent correlation was observed between the two assay results. According to RIA data, the concentration of PAP higher than 0.4 ng per 10 microliter (or 4.0 ng per 100 microliter) signify the elevation of serum or bone marrow PAP level beyond normal range (normal value 1.6 +/- 0.8 ng/100 microliter). Thus, the CIEP assay will be a simple and reliable screening method for the serum PAP levels in the clinical diagnosis of prostatic cancer.
当通过对流免疫电泳（CIEP）将标准前列腺酸性磷酸酶（PAP）的系列稀释液与恒定剂量的抗PAP血清反应时，在10微升样本体积中酶浓度的检测终点为0.25纳克。未知样本中的PAP浓度可通过比较参考PAP与测试样本的稀释终点来定量。使用正常男性和女性血清以及前列腺癌和非前列腺肿瘤患者的血清样本，通过放射免疫测定法（RIA）和CIEP测定血清PAP水平。观察到两种检测结果之间具有良好的相关性。根据RIA数据，每10微升PAP浓度高于0.4纳克（或每100微升4.0纳克）表明血清或骨髓PAP水平升高超出正常范围（正常值为1.6±0.8纳克/100微升）。因此，CIEP检测将是前列腺癌临床诊断中血清PAP水平的一种简单可靠的筛查方法。
