Azocar J, Essex M, Watson A, Gazit E, Anderson D, Yunis E J
Hum Immunol. 1982 Dec;5(4):283-93. doi: 10.1016/0198-8859(82)90020-9.
Changes in the expression of HLA and beta 2-microglobulin (beta 2-m) antigens by cultured human lymphoid cell lines were investigated. HLA expression was assayed by indirect trace binding radioimmunoassay (RIA) with monoclonal antibodies and by determining sensitivity to complement-dependent lysis by alloantisera. Lymphoid cells in culture were found to undergo changes in the expression of HLA and beta 2-m antigens characterized by decreased membrane expression of these antigens at high cell densities or after a prolonged period of cultivation. The decreased expression of HLA and beta 2-m antigens apparently is due neither to a masking phenomenon nor to a lack of nutrients or an accumulation of metabolites in the culture media but is perhaps mediated by a cell-to-cell contact mechanism. Human interferon was found to enhance the expression of HLA and beta 2-m, apparently overriding the effects on major histocompatibility complex (MHC) expression induced by cell density.
研究了培养的人淋巴细胞系中HLA和β2-微球蛋白（β2-m）抗原表达的变化。通过用单克隆抗体的间接微量结合放射免疫测定法（RIA）以及通过测定同种抗血清对补体依赖性溶解的敏感性来检测HLA表达。发现培养中的淋巴细胞会发生HLA和β2-m抗原表达的变化，其特征是在高细胞密度或长时间培养后这些抗原的膜表达降低。HLA和β2-m抗原表达的降低显然既不是由于掩盖现象，也不是由于培养基中缺乏营养物质或代谢产物的积累，而是可能由细胞间接触机制介导。发现人干扰素可增强HLA和β2-m的表达，显然克服了细胞密度对主要组织相容性复合体（MHC）表达的影响。
