Heron I, Hokland M, Berg K
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6215-9. doi: 10.1073/pnas.75.12.6215.
Mononuclear cells from the blood of healthy normal humans were kept in cultures under nonstimulating conditions for 16 hr in the presence or absence of human interferon. The relative quantities of HLA antigens and beta(2)-microglobulin on the cultured cells were determined by quantitative immunofluorescence (fluorescence-activated cell sorter) and by the capacity of cells to absorb out cytotoxic antibodies against the relevant antigens. Interferons of different origin and purities enhanced the expression of HLA antigens and beta(2)-microglobulins, whereas membrane immunoglobulins and antigens recognized by antiserum raised against human brain and T cells were the same on interferon-treated and control cells. Similar interferon effects were observed on an Epstein-Barrvirus-negative Burkitt lymphoma cell line. The enhanced expression of histocompatibility antigen subsequent to intereferon treatment was observed on B- and T-enriched lymphocyte populations and was found to be dose dependent with the optimum with "physiological" concentrations of interferon. Pretreatment of lymphocytes with interferon for 2 hr was found to be as effective as having interferon present during the total culture period. The interferon-induced enhancement of antigen expression on cells was dependent on active protein synthesis.
从健康正常人血液中获取的单核细胞，在有无人类干扰素存在的情况下，于非刺激条件下培养16小时。通过定量免疫荧光法（荧光激活细胞分选仪）以及细胞吸收针对相关抗原的细胞毒性抗体的能力，来测定培养细胞上HLA抗原和β2-微球蛋白的相对含量。不同来源和纯度的干扰素可增强HLA抗原和β2-微球蛋白的表达，而在经干扰素处理的细胞和对照细胞上，膜免疫球蛋白以及针对人脑和T细胞产生的抗血清所识别的抗原是相同的。在爱泼斯坦-巴尔病毒阴性的伯基特淋巴瘤细胞系上也观察到了类似的干扰素效应。在富含B细胞和T细胞的淋巴细胞群体上，观察到干扰素处理后组织相容性抗原表达增强，且发现其具有剂量依赖性，在“生理”浓度的干扰素作用下达到最佳效果。发现用干扰素预处理淋巴细胞2小时与在整个培养期间都存在干扰素一样有效。干扰素诱导的细胞抗原表达增强依赖于活性蛋白质合成。
