Farshy C E, Kennedy E J, Hunter E F, Larsen S A
J Clin Microbiol. 1983 Feb;17(2):245-8. doi: 10.1128/jcm.17.2.245-248.1983.
The fluorescent treponemal antibody absorption (FTA-ABS) double-staining (DS) test has been developed for microscopes equipped with incident illumination, and the procedure offers many advantages over the FTA-ABS test when tests are performed with this equipment. In this study, 346 fresh sera, including 35 from patients with syphilis, were evaluated by the FTA-ABS DS test. Parameters for investigation included two readers, each using a different microscope; a new FTA-ABS DS test reporting system; sera heated at 56 degrees C for 30 min versus unheated sera; and sera retested after at least 2 weeks of freezer storage. Agreement for FTA-ABS DS test readings between the two microscopes was 99%. Between-test agreement for the FTA-ABS test with the conventional reporting system and the FTA-ABS DS test with the new reporting system was 95%. Sensitivity calculations based on reactivity for the 35 syphilis sera were 94% for the FTA-ABS DS test and 91% for the FTA-ABS test. Specificity calculations based on non-reactivity of nonsyphilis sera were 98% for the FTA-ABS DS test and 93% for the FTA-ABS test. Differences in percentages appeared to be related to borderline readings in the FTA-ABS test. For example, if the same reporting system was used for the reference FTA-ABS test, the specificity was 97%. When sera were examined within 48 h, no difference was observed in results obtained with heated and unheated sera. Sera frozen for 2 weeks showed comparable results in the FTA-ABS DS test and the FTA-ABS test. These findings strongly support the recommendation that the FTA-ABS DS test be accepted as a confirmatory test for syphilis. The new reporting system for the FTA-ABS DS test would be advantageous for the reference FTA-ABS procedure.
荧光密螺旋体抗体吸收（FTA - ABS）双染色（DS）试验是为配备落射照明的显微镜开发的，当使用该设备进行检测时，该方法比FTA - ABS试验具有许多优势。在本研究中，通过FTA - ABS DS试验对346份新鲜血清进行了评估，其中包括35份梅毒患者的血清。研究参数包括两名分别使用不同显微镜的阅片者；一种新的FTA - ABS DS试验报告系统；56℃加热30分钟的血清与未加热血清；以及冷冻保存至少2周后的血清重新检测。两台显微镜之间FTA - ABS DS试验读数的一致性为99%。传统报告系统的FTA - ABS试验与新报告系统的FTA - ABS DS试验之间的组间一致性为95%。基于35份梅毒血清反应性的敏感性计算结果为，FTA - ABS DS试验为94%，FTA - ABS试验为91%。基于非梅毒血清无反应性的特异性计算结果为，FTA - ABS DS试验为98%，FTA - ABS试验为93%。百分比差异似乎与FTA - ABS试验中的临界读数有关。例如，如果参考FTA - ABS试验使用相同的报告系统，特异性为97%。在48小时内检测血清时，加热和未加热血清的检测结果未观察到差异。冷冻2周的血清在FTA - ABS DS试验和FTA - ABS试验中显示出可比的结果。这些发现有力地支持了将FTA - ABS DS试验作为梅毒确证试验的建议。FTA - ABS DS试验的新报告系统对参考FTA - ABS程序将是有利的。
