Farshy C E, Hunter E F, Helsel L O, Larsen S A
J Clin Microbiol. 1985 Mar;21(3):387-9. doi: 10.1128/jcm.21.3.387-389.1985.
Further studies of a four-step enzyme-linked immunosorbent assay procedure to detect Treponema pallidum antibody are described. High-titered antibody, produced in rabbits by intravenous injection of T. pallidum, was used to coat polyvinyl chloride microtiter plates. To these plates a known concentration of T. pallidum was added, followed in successive steps by serial dilutions of human sera and appropriately diluted peroxidase-labeled anti-human immunoglobulin G antibody. O-Phenylenediamine was the substrate. A total of 340 sera were obtained from the DeKalb County Sexually Transmitted Diseases Clinic, Atlanta, Ga., and examined within 3 days of receipt. Ninety-six percent test agreement between the enzyme-linked immunosorbent assay and the fluorescent treponemal antibody absorption-double staining test was obtained. A total of 372 additional sera stored at -20 degrees C were examined. The overall sensitivity of the enzyme-linked immunosorbent assay with sera from patients with various stages of syphilis was 96%. With sera from uninfected individuals, the specificity of the enzyme-linked immunosorbent assay was 95%. No antigen instability was noted with the two antigen preparations used during this evaluation.
本文描述了一种用于检测梅毒螺旋体抗体的四步法酶联免疫吸附测定程序的进一步研究。通过静脉注射梅毒螺旋体在兔体内产生的高滴度抗体，被用于包被聚氯乙烯微量滴定板。向这些板中加入已知浓度的梅毒螺旋体，随后依次加入人血清的系列稀释液以及适当稀释的过氧化物酶标记抗人免疫球蛋白G抗体。邻苯二胺作为底物。总共从佐治亚州亚特兰大市迪卡尔布县性传播疾病诊所获取了340份血清，并在收到后3天内进行检测。酶联免疫吸附测定与荧光密螺旋体抗体吸收-双重染色试验之间的检测一致性达到96%。另外对372份保存在-20℃的血清进行了检测。酶联免疫吸附测定对于不同梅毒阶段患者血清的总体敏感性为96%。对于未感染个体的血清，酶联免疫吸附测定的特异性为95%。在该评估过程中使用的两种抗原制剂未发现抗原不稳定现象。
