荧光密螺旋体抗体吸收（FTA-ABS）试验的双重染色程序。
Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.
作者信息
Hunter E F, McKinney R M, Maddison S E, Cruce D D
出版信息
Br J Vener Dis. 1979 Apr;55(2):105-8. doi: 10.1136/sti.55.2.105.
Abstract
The fluorescent treponemal antibody absorption (FTA-ABS) double-staining procedure was reproducible, comparable to the conventional test, and easy to read. We recommend the use of the FTA-ABS double-staining procedure for microscopes with incident illumination, the 100 x/1.30 oil achromatic objective and the 6.3 x ocular to obtain optimal fluorescence, and the KP560 as a barrier filter to exclude rhodamine emission when fluorescein fluorescence is read. With this system, errors related to poor focusing or failure to visualise treponemes on all smears should be eliminated.
摘要
荧光密螺旋体抗体吸收（FTA-ABS）双重染色程序具有可重复性，与传统检测方法相当，且易于判读。我们建议，对于配备落射照明、100x/1.30油浸消色差物镜和6.3x目镜的显微镜，使用FTA-ABS双重染色程序以获得最佳荧光效果，并使用KP560作为阻挡滤光片，在读取荧光素荧光时排除罗丹明发射光。使用该系统，应可消除因聚焦不佳或未能在所有涂片上观察到密螺旋体而导致的误差。
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本文引用的文献
1
Comparative evaluation of commercial precipitating antisera against human IgM and IgM.针对人IgM和IgM的市售沉淀抗血清的比较评估。
J Lab Clin Med. 1970 Dec;76(6):949-60.
2
The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incidental light.一种用于落射光荧光显微镜的、带有可互换二向色镜的垂直照明器的使用。
Z Wiss Mikrosk. 1967 Nov;68(3):129-42.
3
Immunochemical parameters of some commercial conjugates for the fluorescent treponemal antibody-absorption test.用于荧光梅毒螺旋体抗体吸收试验的一些商业结合物的免疫化学参数。
Infect Immun. 1972 Jun;5(6):858-62. doi: 10.1128/iai.5.6.858-862.1972.
4
Immunoglobulin specificity for the fluorescent treponemal antibody-absorption test conjugate.荧光密螺旋体抗体吸收试验结合物的免疫球蛋白特异性。
J Clin Microbiol. 1976 Oct;4(4):338-42. doi: 10.1128/jcm.4.4.338-342.1976.
5
Conjugation methods in immunofluorescence.免疫荧光中的缀合方法。
J Dent Res. 1976 Jan;55:A38-44. doi: 10.1177/002203457605500117011.
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