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劳斯肉瘤病毒转化蛋白pp60src单克隆抗体的分离与部分特性鉴定

Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src.

作者信息

Parsons S J, McCarley D J, Ely C M, Benjamin D C, Parsons J T

出版信息

J Virol. 1983 Mar;45(3):1190-4. doi: 10.1128/JVI.45.3.1190-1194.1983.

DOI:10.1128/JVI.45.3.1190-1194.1983
PMID:6187941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256532/
Abstract

Transformation of cells by Rous sarcoma virus is mediated by the product of the viral src gene, pp60src. A hybridoma cell line producing an immunoglobulin G3 antibody to pp60src was isolated after lymph node cells from immune mice were fused with mouse myeloma cells (P3-NS1-1). Mice were immunized with p60src purified from Escherichia coli cells expressing the src gene product. The monoclonal antibody immunoprecipitated pp60src from Rous sarcoma virus-transformed cells and recognized an antigenic determinant located in the amino-terminal third of the pp60src protein.

摘要

劳氏肉瘤病毒对细胞的转化是由病毒src基因的产物pp60src介导的。用来自免疫小鼠的淋巴结细胞与小鼠骨髓瘤细胞（P3-NS1-1）融合后，分离出了一种产生针对pp60src的免疫球蛋白G3抗体的杂交瘤细胞系。用从表达src基因产物的大肠杆菌细胞中纯化的p60src对小鼠进行免疫。该单克隆抗体从劳氏肉瘤病毒转化的细胞中免疫沉淀出pp60src，并识别位于pp60src蛋白氨基末端三分之一处的一个抗原决定簇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/726d517b7e49/jvirol00150-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/acf424a87e10/jvirol00150-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/a1c0b8811b7b/jvirol00150-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/e17e152081af/jvirol00150-0302-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/726d517b7e49/jvirol00150-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/acf424a87e10/jvirol00150-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/a1c0b8811b7b/jvirol00150-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/e17e152081af/jvirol00150-0302-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f8/256532/726d517b7e49/jvirol00150-0303-a.jpg

相似文献

1
Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src.劳斯肉瘤病毒转化蛋白pp60src单克隆抗体的分离与部分特性鉴定
J Virol. 1983 Mar;45(3):1190-4. doi: 10.1128/JVI.45.3.1190-1194.1983.
2
Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.识别禽肉瘤病毒转化蛋白的单克隆抗体的分离
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Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.针对劳氏肉瘤病毒pp60src的单克隆抗体可与源自禽类和哺乳动物的具有酶活性的细胞pp60src发生反应。
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Monoclonal antibody against the carboxy terminal peptide of pp60src of Rous sarcoma virus reacts with native pp60src.针对劳氏肉瘤病毒pp60src羧基末端肽的单克隆抗体与天然pp60src发生反应。
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Development and characterization of antisera specific for amino- and carboxy-terminal regions of pp60src.pp60src氨基端和羧基端区域特异性抗血清的研制与特性分析
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Immune response to the src gene product in mice bearing tumors induced by injection of avian sarcoma virus-transformed mouse cells.对注射禽肉瘤病毒转化的小鼠细胞诱导产生肿瘤的小鼠体内src基因产物的免疫反应。
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Differences in intracellular location of pp60src in rat and chicken cells transformed by Rous sarcoma virus.劳氏肉瘤病毒转化的大鼠和鸡细胞中pp60src的细胞内定位差异。
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9
Changes in amino-terminal sequences of pp60src lead to decreased membrane association and decreased in vivo tumorigenicity.pp60src氨基末端序列的变化导致膜结合减少和体内致瘤性降低。
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Localization of conserved and nonconserved epitopes within the Rous sarcoma virus-encoded src protein.罗氏肉瘤病毒编码的src蛋白中保守和非保守表位的定位。
J Virol. 1986 Sep;59(3):755-8. doi: 10.1128/JVI.59.3.755-758.1986.

引用本文的文献

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Amino acid alterations within a highly conserved region of the Rous sarcoma virus src gene product pp60src inactivate tyrosine protein kinase activity.劳氏肉瘤病毒src基因产物pp60src高度保守区域内的氨基酸改变会使酪氨酸蛋白激酶活性失活。
Mol Cell Biol. 1984 May;4(5):862-6. doi: 10.1128/mcb.4.5.862-866.1984.
2
Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.识别禽肉瘤病毒转化蛋白的单克隆抗体的分离
J Virol. 1983 Nov;48(2):352-60. doi: 10.1128/JVI.48.2.352-360.1983.
3
Site-directed point mutation in the src gene oF rous sarcoma virus results in an inactive src gene product.

本文引用的文献

1
Development of anti-pp60src serum with antigen produced in Escherichia coli.利用在大肠杆菌中产生的抗原制备抗pp60src血清。
J Virol. 1983 Jan;45(1):462-5. doi: 10.1128/JVI.45.1.462-465.1983.
2
Site-directed mutagenesis of the src gene of Rous sarcoma virus: construction and characterization of a deletion mutant temperature sensitive for transformation.劳氏肉瘤病毒src基因的定点诱变：对转化温度敏感的缺失突变体的构建与特性分析
J Virol. 1982 Nov;44(2):683-91. doi: 10.1128/JVI.44.2.683-691.1982.
3
Construction of plasmids for expression of Rous sarcoma virus transforming protein, p60src, in Escherichia coli.
劳氏肉瘤病毒src基因中的定点诱变导致无活性的src基因产物。
J Virol. 1983 Mar;45(3):1211-6. doi: 10.1128/JVI.45.3.1211-1216.1983.
4
Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.针对劳氏肉瘤病毒pp60src的单克隆抗体可与源自禽类和哺乳动物的具有酶活性的细胞pp60src发生反应。
J Virol. 1984 Aug;51(2):272-82. doi: 10.1128/JVI.51.2.272-282.1984.
5
Isolation of monoclonal antibodies specific for products of avian oncogene myb.针对禽类癌基因myb产物的单克隆抗体的分离
Mol Cell Biol. 1984 Dec;4(12):2843-50. doi: 10.1128/mcb.4.12.2843-2850.1984.
6
Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for cellular transformation.编码具有改变的羧基末端的src蛋白的劳氏肉瘤病毒变体在细胞转化方面存在缺陷。
J Virol. 1985 Aug;55(2):314-21. doi: 10.1128/JVI.55.2.314-321.1985.
7
Altered processing of a major secreted phosphoprotein correlates with tumorigenicity in Rous sarcoma virus-transformed mammalian cells.一种主要分泌磷蛋白的加工改变与劳氏肉瘤病毒转化的哺乳动物细胞中的致瘤性相关。
J Virol. 1985 Mar;53(3):841-50. doi: 10.1128/JVI.53.3.841-850.1985.
8
Inhibition of the tyrosine kinase activity of v-src, v-fgr, and v-yes gene products by a monoclonal antibody which binds both amino and carboxy peptide fragments of pp60v-src.一种结合pp60v-src氨基和羧基肽片段的单克隆抗体对v-src、v-fgr和v-yes基因产物酪氨酸激酶活性的抑制作用
J Virol. 1987 Jun;61(6):1927-37. doi: 10.1128/JVI.61.6.1927-1937.1987.
9
Forms of pp60v-src isolated from Rous sarcoma virus-transformed cells.从劳氏肉瘤病毒转化细胞中分离出的pp60v-src形式。
J Virol. 1987 May;61(5):1593-601. doi: 10.1128/JVI.61.5.1593-1601.1987.
10
Localization of conserved and nonconserved epitopes within the Rous sarcoma virus-encoded src protein.罗氏肉瘤病毒编码的src蛋白中保守和非保守表位的定位。
J Virol. 1986 Sep;59(3):755-8. doi: 10.1128/JVI.59.3.755-758.1986.
用于在大肠杆菌中表达劳氏肉瘤病毒转化蛋白p60src的质粒构建。
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2152-6. doi: 10.1073/pnas.79.7.2152.
4
Evidence the pp60src, the product of the Rous sarcoma virus src gene, undergoes autophosphorylation.有证据表明，劳氏肉瘤病毒src基因的产物pp60src会发生自身磷酸化。
J Virol. 1982 Jan;41(1):1-7. doi: 10.1128/JVI.41.1.1-7.1982.
5
Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus.禽肉瘤病毒Y73的基因组序列及其转化基因产物与劳氏肉瘤病毒转化基因产物的结构相似性。
Nature. 1982 May 20;297(5863):205-8. doi: 10.1038/297205a0.
6
A third class of avian sarcoma viruses, defined by related transformation-specific proteins of Yamaguchi 73 and Esh sarcoma viruses.第三类禽肉瘤病毒，由山口73和埃什肉瘤病毒的相关转化特异性蛋白所定义。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2611-5. doi: 10.1073/pnas.78.4.2611.
7
Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.纽蛋白：劳氏肉瘤病毒转化蛋白的细胞骨架靶点。
Cell. 1981 Apr;24(1):165-74. doi: 10.1016/0092-8674(81)90512-2.
8
Characterization of protein kinase activity associated with the transforming gene product of Fujinami sarcoma virus.与藤浪肉瘤病毒转化基因产物相关的蛋白激酶活性的特性分析
Cell. 1980 Dec;22(3):757-65. doi: 10.1016/0092-8674(80)90552-8.
9
Molecular cloning of avian sarcoma virus closed circular DNA: structural and biological characterization of three recombinant clones.禽肉瘤病毒闭合环状DNA的分子克隆：三个重组克隆的结构与生物学特性
J Virol. 1980 Oct;36(1):271-9. doi: 10.1128/JVI.36.1.271-279.1980.
10
The purified product of the transforming gene of avian sarcoma virus phosphorylates tyrosine.禽肉瘤病毒转化基因的纯化产物使酪氨酸磷酸化。
J Biol Chem. 1980 Dec 25;255(24):11973-80.