Bamford P N, Ormerod M G, Sloane J P, Warburton M J
Obstet Gynecol. 1983 May;61(5):603-8.
Neoplastic and nonneoplastic tissue from human uterine cervixes was stained immunohistochemically for three epithelial antigens--epithelial membrane antigen (EMA), carcinoembryonic antigen (CEA), and cytoplasmic keratin. Anti-EMA serum stained four of 21 nonneoplastic cases and all cases of cervical intraepithelial neoplasia (CIN) (25) and invasive carcinoma (16); CEA was positive in six of ten of the nonneoplastic specimens, 19 of 23 CIN specimens, and six of seven invasive carcinomas tested. Cytoplasmic keratin did not survive fixation in formal saline and for this antigen tissue had to be fixed in methacarn; all the tissues examined were positive.
采用免疫组织化学方法，对取自人子宫颈的肿瘤性和非肿瘤性组织进行三种上皮抗原染色，这三种抗原分别是上皮膜抗原（EMA）、癌胚抗原（CEA）和细胞质角蛋白。抗EMA血清对21例非肿瘤性病例中的4例、所有宫颈上皮内瘤变（CIN，25例）和浸润癌（16例）均呈阳性染色；在检测的10例非肿瘤性标本中，有6例CEA呈阳性，23例CIN标本中有19例呈阳性，7例浸润癌中有6例呈阳性。细胞质角蛋白在甲醛生理盐水中固定后无法保存，对于该抗原，组织必须用甲醇-冰醋酸固定液固定；所有检查的组织均呈阳性。
