Pierson G B, Burton P R, Himes R H
J Cell Biol. 1978 Jan;76(1):223-8. doi: 10.1083/jcb.76.1.223.
Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold-dissociation followed by reassembly in vitro.
使用多种组装条件在体外使来自牛脑的微管蛋白聚合。许多形成的微管显示含有14条壁原纤维。含有14条原纤维的微管数量随着冷解离后连续重复体外重组而增加。