Letchworth G J, Carmichael L E
Vet Microbiol. 1983 Feb;8(1):69-80. doi: 10.1016/0378-1135(83)90020-2.
The production of interferon by bovine peripheral blood leukocytes infected with bovid herpesvirus 2 (BHV-2) was investigated in preparation for studying mechanisms of resistance to BHV-2. It was found that bovine peripheral blood monocytes produced high levels of interferon in response to BHV-2 inoculated at a multiplicity of 1. Virus-induced interferon was not stable at pH 2, was destroyed at 56 degrees C or by incubation with trypsin and was active against both vesicular stomatitis virus and BHV-2. Interferon of high specific activity was produced by incubating monocytes for 5 h with BHV-2 in serum-containing medium, replacing the inoculum with serum-free medium for an additional 16 h, and concentrating the serum-free medium by dialysis against dry polyethylene glycol. Interferon concentrations of 40,000 units per mg of protein were readily attained.
为研究牛对牛疱疹病毒2型（BHV - 2）的抗性机制，对感染该病毒的牛外周血白细胞产生干扰素的情况进行了研究。结果发现，牛外周血单核细胞在接种复数为1的BHV - 2后会产生高水平的干扰素。病毒诱导产生的干扰素在pH 2时不稳定，在56℃下或与胰蛋白酶孵育时会被破坏，并且对水疱性口炎病毒和BHV - 2均有活性。通过在含血清培养基中用BHV - 2孵育单核细胞5小时，然后用无血清培养基替换接种物再孵育16小时，并通过对干燥聚乙二醇进行透析来浓缩无血清培养基，可产生高比活性的干扰素。每毫克蛋白质的干扰素浓度很容易达到40,000单位。
