Fulton R W, Rosenquist B D
Am J Vet Res. 1976 Dec;37(12):1497-502.
The interferon-inducing ability of infectious bovine rhinotracheitis (IBR) virus was determined in tissue cultures of bovine origin inoculated with untreated and ultraviolet (UV) irradiated IBR viruses. Interferon was assayed by the plaque-reduction method in bovine fetal kidney (BFK) cell cultures, using vesicular stomatitis virus as challenge virus. Highest interferon concentrations were produced by cultures of bovine fetal (BF) spleen cells and aveolar macrophage cultures derived from adult cattle. Moderate interferon concentrations were produced by peripheral blood leukocyte (PBL) suspension cultures from adult cattle with serum-neutralizing antibodies against IBR virus. Cultures of PBL from 1 cow without detectable serum-neutralizing antibodies against IBR virus did not produce detectable interferon in response to IBR virus. Cultures of PBL from cattle with or without detectable serum-neutralizing antibodies against IBR virus produced interferon when stimulated with phytohemagglutinin (PHA). Low levles of viral inhibitors were detected infrequently in monolayer cultures of BFK and BF nasal mucosa inoculated with UV-irradiated IBR virus and in BF tracheal organ cultures inoculated with untreated IBR virus. Interferon was not detected in fluids collected from IBR virus-exposed monolayer cultures of primary and secondary BF lung, secondary BF tracheal mucosa, secondary BF liver, secondary BF adrenal, and PBL in the 4th and 7th passages. The antiviral inhibitors from BF spleen, bovine alveolar macrophage, and PBL cultures induced with IBR virus, as well as inhibitors from PBL cultures induced with PHA, had the usual properties of interferon.
在接种未处理的和紫外线（UV）照射的传染性牛鼻气管炎（IBR）病毒的牛源组织培养物中，测定了IBR病毒的干扰素诱导能力。使用水疱性口炎病毒作为攻击病毒，通过噬斑减少法在牛胎肾（BFK）细胞培养物中测定干扰素。最高的干扰素浓度是由牛胎（BF）脾细胞培养物和成年牛的肺泡巨噬细胞培养物产生的。来自具有抗IBR病毒血清中和抗体的成年牛的外周血白细胞（PBL）悬浮培养物产生中等浓度的干扰素。来自1头未检测到抗IBR病毒血清中和抗体的奶牛的PBL培养物在接触IBR病毒时未产生可检测到的干扰素。来自有或没有可检测到的抗IBR病毒血清中和抗体的奶牛的PBL培养物在用植物血凝素（PHA）刺激时产生干扰素。在用紫外线照射的IBR病毒接种的BFK和BF鼻黏膜单层培养物以及用未处理的IBR病毒接种的BF气管器官培养物中，很少检测到低水平的病毒抑制剂。在第4代和第7代原代和二代BF肺、二代BF气管黏膜、二代BF肝、二代BF肾上腺和PBL的IBR病毒暴露单层培养物收集的液体中未检测到干扰素。来自用IBR病毒诱导的BF脾、牛肺泡巨噬细胞和PBL培养物的抗病毒抑制剂，以及用PHA诱导的PBL培养物的抑制剂，具有干扰素的通常特性。
