Ward C W, Murray J M, Roxburgh C M, Jackson D C
Virology. 1983 Apr 15;126(1):370-5. doi: 10.1016/0042-6822(83)90486-5.
The Pronase-released neuraminidase heads from the Asian influenza virus A/Tokyo/3/67 contain four oligosaccharide units attached at asparagine residues 86, 146, 200, and 234. Chemical analysis of the isolated tryptic, chymotryptic, or thermolytic glycopeptides shows that the oligosaccharide side chains attached at residues 86 and 200 are essentially of the oligomannoside (simple or Type II) variety containing two residues of N-acetylglucosamine, five residues of mannose, and less than molar ratios of galactose and fucose. The carbohydrate side chains attached at residues 146 and 234 are of the N-acetyllactosamine (complex or Type I) type and contain N-acetylglucosamine, mannose, galactose, and fucose. The complex oligosaccharide unit at residue 146 is unusual in that it also contains N-acetylgalactosamine, a sugar residue rarely found in N-glycosidically linked carbohydrates. Antigenic analysis of these four isolated glycopeptides showed that only the N-acetyllactosamine oligosaccharide unit at asparagine residue 146 was capable of binding to antibodies raised against uninfected chick chorioallantoic membranes and is hence antigenically related to chick embryo host antigen.
来自亚洲甲型流感病毒A/东京/3/67经链霉蛋白酶释放的神经氨酸酶头部含有四个连接在天冬酰胺残基86、146、200和234上的寡糖单元。对分离出的胰蛋白酶、糜蛋白酶或嗜热菌蛋白酶糖肽的化学分析表明，连接在残基86和200上的寡糖侧链基本上是低聚甘露糖苷（简单型或II型），含有两个N-乙酰葡糖胺残基、五个甘露糖残基，以及低于摩尔比的半乳糖和岩藻糖。连接在残基146和234上的碳水化合物侧链是N-乙酰乳糖胺（复合型或I型）类型，含有N-乙酰葡糖胺、甘露糖、半乳糖和岩藻糖。残基146处的复合寡糖单元不同寻常之处在于它还含有N-乙酰半乳糖胺，这是一种在N-糖苷连接的碳水化合物中很少发现的糖残基。对这四种分离出的糖肽的抗原分析表明，只有天冬酰胺残基146处的N-乙酰乳糖胺寡糖单元能够与针对未感染鸡胚绒毛尿囊膜产生的抗体结合，因此在抗原性上与鸡胚宿主抗原相关。
