Cortay J C, Cozzone A J
Biochem Biophys Res Commun. 1983 May 16;112(3):801-8. doi: 10.1016/0006-291x(83)91688-1.
The interdependency of protein and RNA syntheses was studied comparatively in bacteria confronted with amino acid starvation or treated separately with various aminoglycoside antibiotics. By contrast with the concomitant inhibition of macromolecular syntheses in cells deprived of an essential amino acid, RNA production was found to continue in drug-treated cells while protein synthesis was arrested. Such uncoupling process was also observed in bacteria subjected simultaneously to amino acid starvation and treatment with certain antibiotics (neomycin, gentamicin, spectinomycin and kasugamycin) but not with others (streptomycin and kanamycin). These results were related to the intracellular concentration of guanosine polyphosphates, ppGpp and pppGpp. They were discussed in terms of interaction of aminoglycosides with ribosomes.
在面临氨基酸饥饿的细菌中，或分别用各种氨基糖苷类抗生素处理的细菌中，对蛋白质和RNA合成的相互依赖性进行了比较研究。与缺乏必需氨基酸的细胞中大分子合成的同时抑制相反，发现在药物处理的细胞中RNA产生继续，而蛋白质合成停止。在同时遭受氨基酸饥饿和某些抗生素（新霉素、庆大霉素、壮观霉素和春雷霉素）处理但未用其他抗生素（链霉素和卡那霉素）处理的细菌中也观察到这种解偶联过程。这些结果与鸟苷多磷酸盐、ppGpp和pppGpp的细胞内浓度有关。根据氨基糖苷类与核糖体的相互作用对其进行了讨论。
