Nilheden E, Jeansson S, Vahlne A
J Clin Microbiol. 1983 Apr;17(4):677-80. doi: 10.1128/jcm.17.4.677-680.1983.
We developed a method that uses monoclonal antibodies for typing herpes simplex virus type 1 and type 2 strains. Clinical isolates from GMK cells were seeded directly into a monolayer of GMK cells. After incubation overnight, monoclonal antibodies were added to the infected monolayer, and antibody binding was indicated by a peroxidase enzyme-linked immunosorbent assay. Using prototype strains and previously typed patient strains, we verified the specificity of this technique. This method is now used routinely for typing herpes simplex strains in our laboratory. We have also used this technique for specific staining of type 1 plaques in a mixture of type 1 and type 2 plaques. With this method it is possible to find a single type 1 plaque among several hundred type 2 plaques on a single petri dish. Infectious virus can also be recovered from stained, unfixed type 1 plaques.
我们开发了一种使用单克隆抗体对1型和2型单纯疱疹病毒株进行分型的方法。将来自GMK细胞的临床分离株直接接种到GMK细胞单层中。过夜培养后，将单克隆抗体添加到感染的单层中，并通过过氧化物酶联免疫吸附测定法指示抗体结合。使用原型株和先前分型的患者株，我们验证了该技术的特异性。该方法现在在我们实验室中常规用于单纯疱疹病毒株的分型。我们还使用该技术对1型和2型噬菌斑混合物中的1型噬菌斑进行特异性染色。通过这种方法，可以在单个培养皿上的数百个2型噬菌斑中找到单个1型噬菌斑。感染性病毒也可以从染色的、未固定的1型噬菌斑中回收。
